关键词: 肝炎, 乙型, 慢性, 免疫, T盒21转录因子, 转录因子GATA结合蛋白3, 叉状头/翅膀状螺旋转录因子

Abstract:

Abstract:Objective
To detect the expressions of T-bet,GATA3 and Foxp3 mRNA in peripheral blood mononuclear cells(PBMCs) in patients with chronic hepatitis B(CHB) infection,and to  investigate  the influence in the development of chronic HBV infection.Methods Eighty-six in-patients were selected.According to the diagnostic criteria of CHB  prevention guidelines,they were divided into four groups：33 moderate CHB patients,21 serious CHB patients,16 severe hepatitis patients and 16 cirrhosis patients.10 healthy volunteers were used as controls.RT-PCR method was used to detect the expressions of T-bet,GATA3 and Foxp3 mRNA in PBMCs in patients with CHB.Results The expression levels  of T-bet in moderate CHB group,serious CHB group,severe hepatitis group,cirrhosis group and control group  were  0.597±0.094,0.724±0.071,0.860±0.060,0.528±0.059,and 0.514±0.071,respectively.The  T-bet levels of the patients in serious CHB and severe hepatitis  groups were higher than that in control group(P<0.05);the T-bet level of the patients in moderate CHB group was lower than that in serious CHB group(P<0.05).The expression of  T-bet in serious CHB patients  was lower than that in severe hepatitis group(P<0.05).The levels of GATA3 of the patients in moderate CHB group,serious CHB group,severe hepatitis group,cirrhosis group and control group were 0.966±0.037,0.973±0.021,0.725±0.028,0.759±0.037,and 0.535±0.028,respectively.The levels of GATA3 in patients groups  were  greatly higher than that in control group(P<0.05).The GATA3 expression levels in severe hepatitis group was significantly lower than those in moderate and serious CHB groups(P<0.05).The expression levels of Foxp3 in moderate CHB group,serious CHB group,severe hepatitis group,cirrhosis group and control group were 0.935±0.034,0.919±0.047,0.804±0.021,0.984±0.060,and 0.772±0.083,respectively.The Foxp3 expression level of the patients  in severe group was lower than those in the  other   patients groups(P<0.05).The T-bet/GATA3 ratios in moderate CHB group,serious CHB group,severe hepatitis group,cirrhosis group and control group were 0.618±0.100,0.744±0.072,1.187±0.082,0.697±0.078, and 0.963±0.133, respectively.The T-bet/GATA3 ratio of the patients  in severe hepatitis group was markedly higher than those in the other   patient groups(P<0.05).Conclusion The expressions of T-bet and GATA3 are contrary during the different courses of disease.The ratio of T-bet/GATA3 is negatively correlated with the expression of Foxp3 in the patients with chronic HBV infection.

Key words: hepatitis B,chronic；immunity；T-box 21 transcription factor, GATA-binding protein-3, forkhead box p3