关键词: 骨髓基质干细胞, 骨组织工程, 聚乳酸羟基乙酸/磷酸钙骨水泥, 茜素红染色

Abstract:

Abstract:Objective
To study the feasibility of tissue engineering bone constructed with poly lactic-co-glycolic acid/calcium phosphate cement(PLGA/CPC) and bone marrow stromal cells (BMSCs),and to provide scientific and theoretical and guiding for preventing residual ridge resorption and promoting bone regeneration.Methods Solvent casting - particle leaching technology combined with the phase separation process was used to prepare PLGA/CPC scaffold material;the BMSCs of rats were isolated， cultured, and purified in vitro;the third generation of BMSCs after Dil staining were planted on the composite scaffold material.The experiment was divided into experiment group( PLGA/CPC+BMSCs) and control group(BMSCs).Scanning electron microscope and laser confocal microscope were used to observe the  adhesion of BMSCs on  the PLGA/CPC material;CCK-8 and alkaline phosphatase (AKP) methods were used to detect   the proliferation and differentiation of BMSCs of two materials.Results The porosity of PLGA/CPC scaffold was above 90%,and the average pore diameter was 200-300  μm and the BMSCs were combined with the scaffolds very well；the proliferation of original generation of BMSCs was low during the first 3 d,and it became fast during 3-6 d, but it became low after 6 d;the expressions of  surface antigens CD44 and CD105 in BMSCs were positive;the alizarin red and type Ⅰ collagen staining results showed that  BMSCs had good osteogenic activity;the Dil staining results  showed that the rate of marked cells was     more than 90%,and the markers had no significant effect on the  morphology of BMSCs.The CCK-8 and  AKP results showed  there were no significant differences  of  proliferation rate of BMSCs and AKP activities　between experiment group and control group(P>0.05).Conclusion PLGA/CPC is an ideal tissue engineering scaffold material.

Key words: bone marrow stromal stem cells, bone tissue engineering, poly lactic-co-glycolic acid/calcium phosphate cement, alizarin red staining