吉林大学学报(医学版)

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TFPI-2和MMP-9在食管鳞状细胞癌组织中的表达及其与血管生成拟态的关系

张鲁川,王 峰,樊青霞   

  1. 郑州大学第一附属医院肿瘤内科,河南 郑州  450052
  • 收稿日期:2013-08-14 出版日期:2014-05-28 发布日期:2014-06-05
  • 通讯作者: 樊青霞 (Tel:0371-66295532,E-mail:fqx@2243@sohu.com.cn) E-mail:fqx@2243@sohu.com.cn
  • 作者简介:张鲁川(1985-),男,山东省聊城市人,在读医学硕士,主要从事食管癌血管生成的研究。
  • 基金资助:

    河南省卫生厅医学科技攻关计划项目资助课题(201203011)

Expressions of TFPI-2 and MMP-9 in esophageal squamous cell carcinoma tissue and their relationship with vasculogenic mimicry


ZHANG Lu-chuan,WANG Feng,FAN Qing-xia    

  1. Department of Oncology,  First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052,China
  • Received:2013-08-14 Online:2014-05-28 Published:2014-06-05

摘要:

目的:探讨组织因子途径抑制剂2(TFPI-2)和基质金属蛋白酶9(MMP-9)在食管鳞状细胞癌中的表达及其与血管生成拟态(VM)的关系。方法:收集手术切除的162例食管鳞状细胞癌标本,采用过碘雪夫酸(PAS)和CD34双重染色法观察VM在食管鳞状细胞癌组织中的分布特征。采用免疫组织化学SP法检测TFPI-2和MMP-9在食管鳞状细胞癌组织中的表达情况。分析VM与食管鳞状细胞癌临床病理参数以及TFPI-2和MMP-9表达的关系。结果:食管鳞状细胞癌组织中VM阳性率为20.37%。低分化组VM阳性率(40.38%)高于中分化组(11.76%)和高分化组(7.14%),3组间比较差异有统计学意义(χ2=20.915,P<0.01)。TNM Ⅰ-Ⅱ期组VM阳性率(11.59%)低于Ⅲ期组(26.88%)(χ2=5.707,P=0.017)。在低分化组中,TFPI-2在VM(+)组的表达率[33.34%(7/21)]高于VM(-)组[6.45%(2/31)](χ2=4.582,P=0.032);MMP-9在VM(+)组的表达率[78.79%(26/33)]高于VM(-)组[44.96%(58/129)](χ2=12.05,P=0.001);低分化组TFPI-2的表达水平与VM呈正相关关系(r=0.166,P=0.032),MMP-9的表达水平与VM呈正相关关系(r=0.183,P=0.018),VM(-)组5年生存率高于VM(+)组(χ2=22.84,P<0.001)。结论:低分化及晚期食管鳞状细胞癌易形成VM,VM与食管鳞状细胞癌的不良预后有关,且TFPI-2和MMP-9对VM的形成有促进作用。

关键词: 食管肿瘤, 血管生成拟态, 组织因子途径抑制剂2, 基质金属蛋白酶9

Abstract:

Objective To investigate the expressions of tissue factor pathway inhibitor-2 (TFPI-2) and matrix metalloproteinase-9 (MMP-9) in esophageal squamous c
ell carcinoma(ESCC)tissue and their relationship with vasculogenic mimicry (VM).Methods 162 cases of esophageal squamous cell carcinoma tissues
 were collected.CD34/periodic acid-schiff double staining was performed to observe the distribution of VM in ESCC tissue,and immunohistochemical staining was used to detect the expressions of  TFPI-2 and MMP-9 in ESCC tissue.The relationship between VM  and the clinicopathologic parameters of ESCC,the expressions of TFPI-2 and MMP-9 were analyzed.Results The positive rate of VM in  ESCC tissue was 20.37%.The positive rate of VM in poorly differentiated group(40.38%) was significantly higher than those in moderately differentiated group(11.76%) and well differentiated group(7.14%)(χ2=20.915,P<0.01).The positive rate of VM in TNM Ⅰ-Ⅱ (11.59%) group  was significantly lower than that in TNM Ⅲ group(26.88%)(χ2=5.707,P=0.017).The positive rate of TFPI-2 in VM(+) group(33.34%) was significantly higher than that in VM(-) group(6.45%) (χ2=4.582,P=0.032) in poorly differentiated ESCC. The positive rate of MMP-9 in VM(+) group(78.79%) was significantly higher than that in VM(-) group(44.96%) (χ2=12.05,P=0.001). The expression level of TFPI-2 in poorly differentiated group was positively correlated with  VM(r=0.166,P=0.032),and the expression level of MMP-9 was positively correlated with the VM(r=0.183,P=0.018).The five-year survival rate in VM(-) group was significant higher than that in VM(+) group(χ2=22.84,P<0.001).Conclusion VM exists in ESCC tissue,especially in poorly differentiated and advanced ESCC tissue.VM is related to poor  prognostis of  ESCC.TFPI-2 and MMP-9 might involve in the formation of VM in ESCC.

Key words: esophageal neoplasms, vasculogenic mimicry, tissue factor pathway inhibitor-2, matrix metalloproteinases-9

中图分类号: 

  • R735.1