基底细胞样乳腺癌患者乳腺癌和癌旁正常乳腺组织中MTDH与MMP-9蛋白表达的相关性

doi:10.13481/j.1671-587x.20160321

吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (03): 530-534.doi: 10.13481/j.1671-587x.20160321

基底细胞样乳腺癌患者乳腺癌和癌旁正常乳腺组织中MTDH与MMP-9蛋白表达的相关性

孙亚楠¹, 毛晓韵², 刘晓虹¹, 郭阿垚², 冯亮², 金锋²

1. 中国医科大学附属第一医院普外综合烧伤科, 辽宁沈阳 110001;
2. 中国医科大学附属第一医院乳腺外科, 辽宁沈阳 110001

收稿日期:2016-02-03 发布日期:2016-06-17
通讯作者: 金锋,教授,博士研究生导师(Tel:024-83282618,E-mail:jinfeng66cn@hotmail.com) E-mail:jinfeng66cn@hotmail.com
作者简介:孙亚楠(1982-),女,内蒙古自治区赤峰市人,主治医师,讲师,医学博士,主要从事乳腺癌发生发展分子机制和普通外科手术治疗方面的研究。
基金资助:
国家自然科学基金资助课题(81201886);辽宁省卫计委省直医院科研基金资助课题(LNCCC-D01-2015)

Correlation between expressions of MTDH and MMP-9 proteins in breast cancer and adjacent normal breast tissues of patients with basal- like breast cancer

SUN Yanan¹, MAO Xiaoyun², LIU Xiaohong¹, GUO Ayao², FENG Liang², JIN Feng²

1. Research Unit of General Surgery, Department of Burn Surgery, First Affiliated Hospital, China Medical University, Shenyang 110001, China;
2. Department of Breast Surgery, First Affiliated Hospital, China Medical University, Shenyang 110001, China

Received:2016-02-03 Published:2016-06-17

摘要/Abstract

摘要：

目的: 检测基底细胞样乳腺癌(BLBC)患者乳腺癌组织及配对癌旁正常乳腺组织中异黏蛋白基因(MTDH)与基质金属蛋白酶9(MMP-9)的表达,探讨二者之间的关系。方法: 采用免疫组织化学SP法检测60例BLBC患者癌组织及配对癌旁正常乳腺组织中MTDH和MMP-9蛋白的表达情况;Spearman相关分析法分析二者阳性表达率的相关性。结果: BLBC组织中MTDH和MMP-9阳性表达率均明显高于癌旁配对正常乳腺组织中MTDH和MMP-9的阳性表达率(P<0.05),且其表达与BLBC患者的淋巴结转移、临床分期有关联(P<0.05);有淋巴结转移者癌组织中MTDH和MMP-9阳性表达率高于无淋巴结转移者(χ²=6.89,P<0.05;χ²=15.22,P<0.01);Ⅲ+Ⅳ期BLBC患者癌组织中MTDH和MMP-9蛋白阳性表达率高于Ⅰ+Ⅱ期者(χ²=6.431,P<0.05;χ²=8.014,P<0.05)。BLBC患者癌组织中MTDH和MMP-9的表达呈正相关关系(r=0.531,P<0.01)。结论: BLBC患者癌组织中MTDH与MMP-9蛋白表达有相关性,二者在BLBC形成和进展过程中可能起重要作用。

关键词: 乳腺肿瘤, 基底细胞样乳腺肿瘤, 异黏蛋白, 基质金属蛋白酶9

Abstract:

Objective: To detect the expressions of metadherin (MTDH) and matrix metalloproteinase-9 (MMP-9) in breast cancer and paired adjacent normal breast tissues of the patients with basal-like breast carcinoma (BLBC) and to analyze their correlation. Methods: The expressions of MTDH and MMP-9 in breast cancer and paired adjacent normal breast tissues of 60 BLBC patients were detected by immunohistochemistry method.Pearman analysis was used to detect the correlation between the positive expression rates of MTDH and MMP-9. Results: The positive expression rates of MTDH and MMP-9 in breast cancer tissue were higher than those in paired adjacent normal breast tissue(P<0.05).The expressions of MTDH and MMP-9 in breast cancer tissue were positively correlated with lymph node metastasis and TNM staging of the BLBC patients (P<0.05).The positive expression rates of MTDH and MMP-9 in the BLBC patients with positive lymph node metastasis were much higher than those in the BLBC patients without lymph node metastasis (χ²=6.89,P<0.05;χ²=15.22,P<0.01);the positive expression rates of MTDH and MMP-9 in the BLBC patients in Ⅲ+Ⅳ stage were much higher than those of the BLBC patients in Ⅰ+Ⅱ stage(χ²=6.431,P<0.05;χ²=8.014,P<0.05).There was a positive correlation between the expression of MTDH and the expression of MMP-9 in breast cancer tissue of the BLBC patients (r=0.531,P<0.01). Conclusion: The expression of MTDH is positive correlated with the expression of MMP-9 in breast cancer tissue of the BLBC patients;furthermore,MTDH and MMP-9 protein may play an important role in the carcinogenesis and development of BLBC.

Key words: breast neoplasms, basal-like breast carcinoma, metadherin, matrix metalloproteinase 9

中图分类号:

R737.9

孙亚楠, 毛晓韵, 刘晓虹, 郭阿垚, 冯亮, 金锋. 基底细胞样乳腺癌患者乳腺癌和癌旁正常乳腺组织中MTDH与MMP-9蛋白表达的相关性[J]. 吉林大学学报(医学版), 2016, 42(03): 530-534.

SUN Yanan, MAO Xiaoyun, LIU Xiaohong, GUO Ayao, FENG Liang, JIN Feng. Correlation between expressions of MTDH and MMP-9 proteins in breast cancer and adjacent normal breast tissues of patients with basal- like breast cancer[J]. Journal of Jilin University Medicine Edition, 2016, 42(03): 530-534.

参考文献

[1] Yang L,Parkin DM,Ferlay J,et al.Estimates of cancer incidence in China for 2000 and projections for 2005[J].Cancer Epidemiol Biomarkers Prev,2005,14(1):243-250.

[2] Liu X,Zhang N,Li X,et al.Identification of novel variants of metadherin in breast cancer[J].PLoS One,2011,6(3):e17582.

[3] Yoo BK,Gredler R,Vozhilla N,et al.Identification of genes conferringresistance to 5-fluorouracil[J].Proc Natl Acad Sci USA,2009,106(31):12938-12943.

[4] 陈应超,王玮,李兆龙,等.MTDH和MMP-9在喉鳞状细胞癌组织中的表达及意义[J].临床耳鼻咽喉头颈外科杂志,2016,30(1):60-63.

[7] 周炳娟,张金库,赵文明,等.基底细胞样乳腺癌中CIP2A及 c-myc蛋白表达及意义[J].临床与实验病理学杂志,2012,28(9):966-969.

[8] Perou CM,Srlie T,Eisen MB,et al.Molecular portrails of human breast tumors[J].Nature,2000,406(6797):747-752.

[9] Shi X,Wang X.The role of MTDH/AEG-1 in the progression of cancer[J].Clin Exp Med,2015,8(4):4795-4807.

[10] Li B,Liu Z,Xie X,et al.Primary application of a real-time quantitative polymerase chain reaction for the detection of human breast cancer related novel gene-Metadherin expression[J].Clin-Ger J Clin Oncol, 2010,9(6):316-320.

[11] Emdad L,Das SK,Dasgupta S,et al.AEG-1/MTDH/LYRIC:signaling pathways,downstream genes,interacting proteins,and regulation of tumor angiogenesis[J].Adv Cancer Res,2013,120:75-111.

[12] Hu GH,Wei Y,Kang YB.The multifaceted role of MTDH/AEG-1 in cancer progression[J].Clin Cancer Res,2009,15(18):5615-5620.

[13] 张伟,方子乔,吉化春,等.结肠腺癌组织MTDH和MMP-9表达临床意义研究[J].中华肿瘤防治杂志,2015,22(10):786-795.

[14] 秦瑞英,夏永华,任艳芳,等.AEG-1表达下调对子宫颈癌细胞细胞周期和侵袭能力的影响及其机制[J].中国病理生理杂志,2013,29(6):1020-1030.

[15] Liu L,Wu J,Ying Z,et al.Astrocyte elevated gene-1 upregulates matrix metalloproteinase-9 and induces human glioma invasion[J].Cancer Res,2010,70(9):3750-3759.

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基底细胞样乳腺癌患者乳腺癌和癌旁正常乳腺组织中MTDH与MMP-9蛋白表达的相关性

Correlation between expressions of MTDH and MMP-9 proteins in breast cancer and adjacent normal breast tissues of patients with basal- like breast cancer

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