关键词: 瞬时受体电位通道蛋白6, 乳腺肿瘤, 肿瘤侵袭

Abstract:

Abstract:Objective To explore the expression of transient receptor potential channel 6(TRPC6) in human breast cancer cells, and to clarify the correlation of TRPC6 with  the invasion potential of breast cancer cells.Methods The human breast cancer cell strains MCF-7(hypo-invasion group) and MDA-MB-231(hyper-invasion group)  were cultured.The expressions of TRPC6mRNA and protein in in two groups were detected by RT-PCR and Western blotting methods.Then the MDA-MB-231 cells were divided into control group and SKF96365 group，the effects of SKF96365 on the invasion ability of MDA-MB-231 cells in vitro were explored by wound healing assay and Transwell experiment.Results The results of Western blotting and RT-PCR showed that the  expression  levels of TRPC6 mRNA and  protein   in MDA-MB-231 cells were higher than that in MCF-7 cells（P<0.05).The wound healing assay showed the numbers of migrating cells in 5,25 and 40  μmol/L SKF96365 groups (76.24±7.54，45.33±4.50，25.12±1.57) were lower than those in control group (130.48±9.55) （P<0.05).The Transwell experiment results indicated that the invasiveness of MDA-MB-231 cells were inhibited significantly by SKF96365 compared with control group (P<0.05).Conclusion  The invasion ability of human breast cancer MDA-MB-231 cells is promoted by upregulating the TRPC6 expression，which indicates that the TRPC6 may play role in the metastasis of human breast cancer.

Key words: transient receptor potential channel 6, breast neoplasms, tumor invasion