吉林大学学报(医学版)

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甲状腺乳头状癌患者肿瘤组织和血清中miR-21表达水平检测及其意义

赵民学1,李柏文1,朱 琳1,叶洪珊1,田晓丰2,魏 亮2,曹 宏2   

  1. 1.吉林医药学院附属医院普外科,吉林 吉林132013;2.吉林大学中日联谊医院普外3科,吉林 长春130033
  • 收稿日期:2014-08-17 出版日期:2014-11-28 发布日期:2015-01-18
  • 通讯作者: 曹 宏(Tel:0431-84997753,E-mail:caohong1967@163.com) E-mail:caohong1967@163.com
  • 作者简介:赵民学(1981-),男,吉林省吉林市人,主治医师,医学硕士,主要从事甲状腺和肝胆疾病的基础及临床研究。
  • 基金资助:

    国家自然科学基金资助课题(30872480)

Detection of miR-21 expression levels in carcinoma tissue and serum of patients with papillary thyroid carcinoma and its  significance

ZHAO Min-xue1,LI Bai-wen1,ZHU Lin1,YE Hong-shan1,TIAN Xiao-feng2,WEI Liang2,CAO Hong2   

  1. 1.Department of General Surgery,Affiliated Hospital, Jilin Medical College,Jilin 132013, China;2.Department of General Surgery,China-Japan Uinon Hospital,  Jilin University,Changchun 130033,China
  • Received:2014-08-17 Online:2014-11-28 Published:2015-01-18

摘要:

目的:检测甲状腺乳头状癌(PTC)患者肿瘤组织和血清中miR-21的表达情况,并阐明其临床意义。方法:选择46例PTC患者、35例结节性甲状腺肿患者和18名正常体检者作为研究对象。采用Real-time  PCR法检测研究对象甲状腺肿瘤组织(正常人为甲状腺组织)和血清中miR-21表达水平并进行比较。分析PTC患者肿瘤组织和血清中miR-21表达水平与临床病理特征的关联性。结果:PTC患者肿瘤组织和血清中miR-21相对表达水平为2.74±1.53和0.21±0.08,明显高于结节性甲状腺肿患者(0.92±0.81和0.08±0.05)和正常人(0.86±0.39和0.07±0.03) (P<0.05)。PTC患者肿瘤组织和血清中miR-21的表达与腺外侵润、远处转移、淋巴结转移和不同TNM分期有关联(P<0.05),与性别和肿瘤大小无关联。结论:miR-21在PTC患者肿瘤组织和血清中的表达水平升高,其表达水平与PTC临床病理特征有关联;PTC肿瘤组织和血清中miR-21的检测可能用于PTC临床诊断。

关键词: 甲状腺肿瘤;miR-21;甲状腺肿, 聚合酶链式反应

Abstract:

Abstract:Objective To investigate the expressions of miR-21 in carcinoma tissue and serum of the patients with papillary thyroid carcinoma (PTC),and to clarify its clinical significance.Methods Real-time PCR was used to detect the expression levels of miR-21 in carcinoma tissue and serum of 46 PTC patients,thyroid tissue and serum of  35 cases of nodular goiter and 18 healthy people.The correlations of miR-21 expression level with the clinicopathological features of PTC patients were analyzed.Results The relative expression levels of miR-21 in carcinoma tissue and serum of the PTC patients were 2.74±1.53 and 0.21±0.08,and they were 0.92±0.81 and 0.08±0.05 in carcinoma tissue and serum of the patients with nodular goiter,0.86±0.39 and 0.07±0.03 in normal papillary thyroid tissue and serum of 18 healthy people.The expression levels of miR-21 in carcinoma tissue and serum of the patients with PTC were higher than those of the patients with nodular goiter and healthy people (P<0.05).The expression level of miR-21 in serum and carcinoma tissue of PTC patients had a positive correlation with the extraglandular invasion,distant metastasis,lymph node metastasis, and TNM stages (P<0.05);and it had no correlation with gender and tumor sizes.Conclusion The expression levels of miR-21 in carcinoma tissue and serum of the PTC patients are increased.The up-regulated expression of serum miR-21 is correlated with the clinicopathological features of PTC patients,which indicates that miR-21 could be used as a potential marker for early detection of PTC.

Key words: thyroid neoplasms, mR-21, nodular goiter, polymerase chain reaction

中图分类号: 

  • R736.1