关键词: 黄芪, FLT3受体相互作用凝集素, 细胞增殖

Abstract:

Objective To extract and purify the Fms-like tyrosine kinase-3(FLT3) receptor interacting lectin (FRIL) from Astragalus root, and to analyze and identify its bioactivity. Methods Affinity chromatography was used to purify the crude extraction of Astragalus root;the concentration of FRIL was determined using BCA assay;the property of FRIL combined with sugar was verified using inhibition lectin reaction;the Raji cells were cultured and divided into control, 20 mg·L^-1and 40 mg·L^-1FRIL groups;the cultured HL-60 cells were grouped into 0, 2.5, 5.0, 10.0, 20.0, and 40.0 mg·L^-1 FRIL groups;the proliferation effect of FRIL on human Raji cells and HL-60 cells were measured using flow cytometry and MTT method. Results The 27 mg of FRIL containing 4 subunits was obtained from Astragalus root, which had a high affinity for combining α-D-manoside.The apoptotic rate of Raji cells in 40 mg·L^-1 FRIL group was lower than that in 20 mg·L^-1 FRIL group(P<0.01).Compared with control group, the proliferation activities of the HL-60 cells expressing FLT3 receptors in 5.0-40.0 mg·L^-1 FRIL groups were increased (P<0.05). Conclusion The FRIL protein possessing lectin activity is successfully isolated from Astragalus root, which has the promotion effect on the growth of the cells expressing FLT3 receptor.

Key words: astragalus root, FLT3 receptor interacting lectin, cell proliferation