吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (01): 6-9.doi: 10.13481/j.1671-587x.20150102

• 基础研究 • 上一篇    下一篇

PEDF对肺鳞状细胞癌SK-MES-1细胞增殖、侵袭和凋亡的影响

马建梅1, 陈承2, 李雪萍1   

  1. 1. 西安医学院临床医学院临床技能学教研室, 陕西 西安 710021;
    2. 陕西省西安市第一医院眼科, 陕西 西安 710002
  • 收稿日期:2014-08-05 发布日期:2015-01-30
  • 通讯作者: 李雪萍,教授,硕士研究生导师(Tel:029-86168677,E-mail:lxp_lucy@qq.com);陈承,主治医师(Tel:029-87630864,E-mail:cc406101098@163.com) E-mail:lxp_lucy@qq.com;cc406101098@163.com
  • 作者简介:马建梅(1976-),女,河北省交河县人,讲师,医学硕士,主要从事呼吸系统疾病和肺癌防治方面的研究。
  • 基金资助:

    国家自然科学基金资助课题(81100665)

Effects of PEDF on proliferation,invasion and apoptosis of lung squamous cell carcinoma SK-MES-1 cells

MA Jianmei1, CHEN Cheng2, LI Xueping1   

  1. 1. Department of Clinical Skills, College of Clinical Medicine, Xi'an Medical University, Xi'an 710021, China;
    2. Department of Ophthalmology, First Hospital, Xi'an City, Shanxi Province, Xi'an 710002, China
  • Received:2014-08-05 Published:2015-01-30

摘要:

目的: 探讨色素上皮衍生因子(PEDF)对肺鳞状细胞癌(鳞癌)SK-MES-1细胞增殖、凋亡和侵袭的影响,阐明PEDF与肺鳞癌发生发展的关系。方法: 培养SK-MES-1细胞,设不加PEDF的细胞为阴性对照组,PEDF干预组细胞分别加入180、360和720 nmol·L-1 PEDF;MTT法检测各组SK-MES-1细胞增殖抑制率,细胞侵袭实验测定阴性对照组和720 nmol·L-1 PEDF干预组SK-MES-1细胞穿透数,倒置显微镜观察各组SK-MES-1细胞形态学,流式细胞术检测各组SK-MES-1细胞凋亡率。结果: PEDF干预组细胞增殖抑制率均显著高于阴性对照组(P<0.01),不同剂量PEDF干预组间比较差异也有统计学意义(P<0.05)。阴性对照组细胞贴壁生长良好,PEDF干预组细胞排列逐渐稀疏,形态改变明显。720 nmol·L-1 PEDF干预组SK-MES-1细胞穿透数(31.6±15.8)低于阴性对照组(75.4±19.3),差异有统计学意义(P<0.01)。随着PEDF干预剂量增加,细胞凋亡率逐渐升高,不同剂量PEDF干预组与阴性对照组比较差异均有统计学意义(P<0.05)。结论: PEDF抑制SK-MES-1细胞增殖和侵袭,促进细胞凋亡,对肺鳞癌生长和转移发挥对抗效应。提示PEDF可能成为肺鳞癌患者预后判定、抗癌治疗的有效因子。

关键词: 色素上皮衍生因子, 肺肿瘤, SK-MES-1细胞, 细胞增殖, 侵袭, 细胞凋亡

Abstract:

Objective To explore the effects of pigment epithelium derived factor(PEDF) on the proliferation, apoptosis and invasion of lung squamous cell carcinoma SK-MES-1 cells, and to interpret the relationships between PEDF and the carcinogenesis and progression of lung squamous cell carcinoma. Methods The SK-MES-1 cells were primarily cultured.The cells without PEDF were usded as negative control group, the cells in PEDF intervention groups were treated with 180, 360, and 720 nmol·L-1 PEDF.The inhibitory rates of proliferation of SK-MES-1 cells were observed by MTT assay, the number of SK-MES-1 cells penetrating the membrane in negative control group and 720 nmol·L-1 PEDF group were determined by cell invasion experiment, the morphology of SK-MES-1 cells was observed by inverted microscope, and the apoptotic rates were detected by flow cytometry. Results The inhibitory rates of proliferation of SK-MES-1 cells in PEDF intervention groups were higer than that in negative control group(P<0.01), and there were statistics differences among the PEDF groups(P<0.05).The cells in negative control group grew well, but the morphological changes of the cells in intervention groups were obvious.The number of the cells penetrating the membrane in 720 nmol·L-1 PEDF intervention group (31.6±15.8) was lower than that in negative control group (75.4±19.3)(P<0.01).The apoptotic rates of SK-MES-1 cells were increased with the increasing of PEDF doses, and there were significant differences between different doses of PEDF intervention groups and negative control group (P<0.05). Conclusion PEDF inhibits the proliferation or invasion and promotes the apoptosis of SK-MES-1 cells, and play an effect of inhibiting the growth and metastasis of lung squamous cell carcinoma.PEDF may be a potent factor for the prognosis and treatment of lung squamous cell carcinoma.

Key words: pigment epithelium derived factor, lung neoplasms, SK-MES-1 cells, cell proliferation, invasion, apoptosis

中图分类号: 

  • R734.2