过氧化氢通过内质网应激信号通路对Hela细胞自噬和凋亡的诱导作用

doi:10.13481/j.1671-587x.20150301

吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (03): 437-441.doi: 10.13481/j.1671-587x.20150301

• 基础研究 • 下一篇

过氧化氢通过内质网应激信号通路对Hela细胞自噬和凋亡的诱导作用

于洋¹, 徐冶¹, 刘师兵¹, 衣丹², 李松岩¹, 王晓军¹

1. 吉林医药学院医学科研实验室, 吉林吉林 132013;
2. 吉林省吉林中西医结合医院体检中心, 吉林吉林 132010

收稿日期:2014-10-22 出版日期:2015-05-28 发布日期:2015-08-01
通讯作者: 徐冶,教授,硕士研究生导师(Tel:0432-64560371,E-mail:xuye_9707@163.com) E-mail:xuye_9707@163.com
作者简介:于洋(1980-),女,吉林省吉林市人,讲师,医学硕士,主要从事预防医学卫生毒理学方面的研究。
基金资助:
国家自然科学基金面上项目资助课题(81372793);吉林省科技厅自然科学基金资助课题(201015240);吉林省教育厅"十二五"科研项目资助课题(吉教科合字[2013]第361号,[2014]第552号)

Introduction effect of hydrogen peroxide on autophagy and apoptosis in Hela cells through endoplasmic reticulum stresssignal pathway

YU Yang¹, XU Ye¹, LIU Shibing¹, YI Dan², LI Songyan¹, WANG Xiaojun¹

1. Medical Research Laboratory, Jilin Medical College, Jilin 132013, China;
2. Medical Examination Center, Jilin Traditional Chinese and Western Medicine Hospital, Jilin 132010, China

Received:2014-10-22 Online:2015-05-28 Published:2015-08-01

摘要/Abstract

摘要：

目的:利用过氧化氢(H₂O₂)构建人宫颈癌Hela细胞体外氧化应激模型,观察其对Hela细胞自噬和凋亡的影响,并探讨H₂O₂与内质网应激(ERS)信号通路之间的关系。方法:以1 mmol·L^-1 H₂O₂构建体外氧化应激模型;实验分为对照组、H₂O₂ 4 h组、H₂O₂ 8 h组和H₂O₂ 12 h组,各组细胞分别以H₂O₂作用0、4、8及12 h后,利用Western blotting法检测各组Hela细胞中ERS及凋亡、自噬相关蛋白表达。结果:与对照组比较,各H₂O₂组Hela细胞中Cleaved Caspase-3表达水平升高(P<0.05),Cleaved Caspase-4表达水平亦明显升高(P<0.05);各H₂O₂ 组Hela细胞中LC3-Ⅱ表达水平高于对照组(P<0.05),GRP78表达水平亦高于对照组(P<0.05);随着H₂O₂作用时间的延长,各H₂O₂组Hela细胞中IRE1a表达水平明显升高(P<0.01),p-JNK表达水平明显高于对照组(P<0.05)。结论:在H₂O₂所构建的体外氧化应激模型中H₂O₂能够诱导Hela细胞发生自噬和凋亡,且该诱导作用由ERS信号通路介导。

关键词: 过氧化氢, 氧化应激, 内质网应激, 细胞凋亡, 自噬, Hela细胞

Abstract:

Objective To establish a superoxiaized model in vitro by using H₂O₂ in Hela cells and to observe the effect of H₂O₂ on the autophagy and apoptosis in Hela cells, and to investigate the relationship between H₂O₂ and endoplasmic reticulum stress(ERS) signal pathway. Methods 1 mmol·L^-1 H₂O₂ was employed to establish in vitro oxidative stress model.The experiment included control group, H₂O₂ 4 h group, H₂O₂ 8 h group, and H₂O₂ 12 h group;the Hela cells in various groups were treated with H₂O₂ for 0, 4, 8, and 12 h, respectively.The expression levels of apoptotic and autophagic proteins in the Hela cells in various groups were detected by Western blotting method. Results Compared with control group, the expression levels of Cleaved Caspase-3 in H₂O₂ groups were significantly increased (P<0.05), and the expression levels of Cleaved Caspase-4 were increased also(P<0.05).The expression levels of LC3-Ⅱ in H₂O₂ groups were also increased significantly compared with control group(P<0.05).The expression levels of GRP78 in H₂O₂ groups were increased compared with control group(P<0.05), and the expression levels of IRE1a(P<0.01) and p-JNK(P<0.05) were higher than those in control group. Conclusion H₂O₂ can induce the autophagy and apoptosis in Hela cells of in vitro oxidative stress model and its possible mechanism is related to ERS signal pathway.

Key words: hydrogen peroxide, oxidative stress, endoplasmic reticulum stress, apoptosis, autophagy, Hela cells

中图分类号:

R737.3

于洋, 徐冶, 刘师兵, 衣丹, 李松岩, 王晓军. 过氧化氢通过内质网应激信号通路对Hela细胞自噬和凋亡的诱导作用[J]. 吉林大学学报(医学版), 2015, 41(03): 437-441.

YU Yang, XU Ye, LIU Shibing, YI Dan, LI Songyan, WANG Xiaojun. Introduction effect of hydrogen peroxide on autophagy and apoptosis in Hela cells through endoplasmic reticulum stresssignal pathway[J]. Journal of Jilin University Medicine Edition, 2015, 41(03): 437-441.

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过氧化氢通过内质网应激信号通路对Hela细胞自噬和凋亡的诱导作用

Introduction effect of hydrogen peroxide on autophagy and apoptosis in Hela cells through endoplasmic reticulum stresssignal pathway

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