吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (04): 790-793.doi: 10.13481/j.1671-587x.20150423

• 基础研究 • 上一篇    下一篇

TGF-β1对人舌鳞状细胞癌上皮-间质转化的诱导作用及其对PI3K/AKT信号通路的影响

符良斌1, 廖天安1, 王鸿1, 邓伟1, 周斌2, 王友元2   

  1. 1. 海南省人民医院口腔颌面外科, 海南 海口 570001;
    2. 中山大学孙逸仙纪念医院口腔颌面外科, 广东 广州 510120
  • 收稿日期:2014-01-23 发布日期:2015-08-01
  • 通讯作者: 王友元,主治医师(Tel:020-81332220,E-mail:sumswangyy@163.com) E-mail:sumswangyy@163.com
  • 作者简介:符良斌(1981-),男,海南省临高县人,主治医师,医学硕士,主要从事口腔癌的基础和治疗方面的研究。
  • 基金资助:

    广东省卫生厅医学科研基金资助课题(B2014136)

Induction effect of TGF-β1 on epithelial-to-mesenchymal transition of human tongue squamous cell carcinoma and its influence in PI3K/AKT signaling pathway

FU Liangbin1, LIAO Tianan1, WANG Hong1, DENG Wei1, ZHOU Bin2, WANG Youyuan2   

  1. 1. Department of Oral and Maxillofacial Surgery, Hainan Provincial People's Hospital, Haikou 570001, China;
    2. Department of Oral and Maxillofacial Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China
  • Received:2014-01-23 Published:2015-08-01

摘要:

目的: 研究转化生长因子β1(TGF-β1)诱导人舌鳞状细胞癌(鳞癌)细胞上皮-间质转化(EMT)的作用,并探讨其对PI3K/AKT信号通路的影响。方法: 体外培养的舌鳞癌细胞SCC9和CAL27分为对照组和不同浓度TGF-β1(5和10 μg·L-1)组,TGF-β1刺激24 h后,光学显微镜下观察各组细胞形态学,细胞免疫荧光实验检测细胞中间质相关标记蛋白Vimentin和上皮相关标记蛋白E-cadherin的表达,Western blotting法检测细胞中AKT和p-AKT的表达。结果: 与对照组比较,TGF-β1组舌鳞癌SCC9和CAL27细胞形态从多边形上皮样结构、成簇生长、细胞之间连接紧密状态变为单个分散的长梭形细胞,细胞间连接消失,呈现间质细胞表型。与对照组比较,TGF-β1组SCC9和CAL27细胞中Vimentin表达强度升高,E-cadherin 表达降低;与对照组比较,TGF-β1组SCC9和CAL27细胞中p-AKT表达强度降低。结论: TGF-β1可诱导舌鳞癌细胞发生EMT,并影响PI3K/AKT信号通路。

关键词: 转化生长因子&beta, 1, 舌肿瘤, 上皮间质转化, PI3K/AKT

Abstract:

Objective To investigate the effects of transforming growth factor β1 (TGF-β1) on the epithelial-to-mesenchymal transition(EMT)in human tongue squamous cell carcinoma, and to explore its influence in PI3K/AKT signaling pathway. Methods The in vitro cultured tongue squamous cell carcinoma SCC9 cells and CAL27 cells were divided into control group and different concentrations (5 and 10 μg·L-1)of TGF-β1 groups;24 h after TGF-β1 treatment,the morphology of cells in various groups were observed under light microscope. Immunoflurescence staining was used to detect the expressions of Vimentin and E-cadherin in the cells in various groups. The expressions of AKT and p-AKT in the SCC9 and CAL27 cells were measured by Western blotting method. Results Compared with control group,the SCC9 and CAL27 cells with polygonal morphology trans-differentiated into mesenchymal phenotype with an elongated morphology in TGF-β1 groups.Compared with control group,the expressions of Vimentin protein in the cells in TGF-β1 groups were increased and the expressions of E-cadherin were decreased. Compared with control group,the expression levels of p-AKT were decreased in SCC9 and CAL27 cells in TGF-β1 groups. Conclusion TGF-β1 can induce the EMT in human tongue squamous cell carcinoma and affects the PI3K/AKT signaling pathway.

Key words: transforming growth factor β1, tongue neoplasms, epithelial-to-mesenchymal transition, PI3K/AKT

中图分类号: 

  • R739.8