P21和survivin蛋白在脑胶质瘤组织中的表达及其临床意义

doi:10.13481/j.1671-587x.20150523

吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (05): 1004-1007.doi: 10.13481/j.1671-587x.20150523

P21和survivin蛋白在脑胶质瘤组织中的表达及其临床意义

董志强, 李强, 贺振华, 潘亚文

兰州大学第二医院神经外科, 甘肃兰州 730030

收稿日期:2015-01-14 发布日期:2015-09-29
通讯作者: 潘亚文,主任医师,教授,硕士研究生导师(Tel:0931-8943720,E-mail:panyawen666@souhu.com) E-mail:panyawen666@souhu.com
作者简介:董志强(1977-),男,甘肃省陇西县人,主治医师,医学博士,主要从事颅脑肿瘤和颅脑损伤的基础和临床方面的研究。
基金资助:
中国博士后科学基金会科研项目资助课题(2013M542395)

Expressions of P21 and survivin proteins in glioma tissue and their clinical significances

DONG Zhiqiang, LI Qiang, HE Zhenhua, PAN Yawen

Department of Neurosurgery, Second Hospital, Lanzhou University, Lanhzou 730030, China

Received:2015-01-14 Published:2015-09-29

摘要/Abstract

摘要：

目的:探讨不同级别人脑胶质瘤组织和正常脑组织中P21和survivin蛋白的表达情况,分析其临床意义。方法:按2007年WHO制定的病理分级标准,将41份脑胶质瘤组织标本分为Ⅰ级9份、Ⅱ级14份、Ⅲ级10份和Ⅳ级8份,另外12份正常脑组织作为对照组,采用免疫组织化学法检测脑胶质瘤和正常脑组织中P21和survivin蛋白的表达情况,并分析两者表达的关系。结果:在正常脑组织和脑胶质瘤组织中P21及survivin蛋白阳性表达率分别为91.7%和53.7%及0%和51.2%,不同组织中P21和survivin蛋白的阳性表达率比较差异均有统计学意义(P<0.05)。Ⅰ-Ⅱ和Ⅲ-Ⅳ级脑胶质瘤组织中P21蛋白阳性表达率分别为69.6％及33.3%,而survivin蛋白阳性表达率分别为34.8%和72.2%,Ⅲ-Ⅳ级脑胶质瘤组织中P21蛋白阳性表达率明显低于Ⅰ-Ⅱ级,而survivin蛋白的阳性表达率明显高于Ⅰ-Ⅱ级(P<0.05)。结论:P21蛋白的表达缺失和survivin蛋白的过度表达与脑胶质瘤恶性程度有关联,P21蛋白有抑制脑胶质瘤发生发展的作用,而survivin蛋白在脑胶质瘤的恶变过程中起促进作用。

关键词: 脑胶质瘤, P21, survivin, 免疫组织化学

Abstract:

Objective To investigate the expressions of P21 and survivin proteins in human glioma tissue, and normal brain tissue, and to analyze their clinical significances. Methods According to the pathological standards formulated by the WHO in 2007,41 cases of glioma tissue samples were divided into 9 cases of grade Ⅰ,14 cases of gradeⅡ,10 cases of grade Ⅲ and 8 cases of grade Ⅳ;the other 12 cases of normal brain tissue were used as control group.The expressions of P21 and survivin proteins in human glioma tissue and normal brain tissue were examined with immunohistochemical method,and the relationship between them was analyzed. Results The positive expression rates of P21 and survivin proteins in normal brain tissue and glioma tissue were 91.7% and 53.7%,0% and 51.2%,and the differences between different tissues were statistically significant (P<0.05).The positive expression rates of P21 protein in the grade Ⅰ-Ⅱ and grade Ⅱ-Ⅲ glioma tissue were 69.6% and 33.3%,respectively,while the positive expression rates of survivin protein were 34.8% and 72.2%.The positive expression rate of P21 in the grade Ⅲ-Ⅳ glioma tissue was significantly lower than that in the grade Ⅰ-Ⅱglioma tissue, while the positive expression rate of survivin protein in the grade Ⅲ-Ⅳ glioma tissue was significantly higher than that in the grade Ⅰ-Ⅱ glioma tissue(P<0.05). Conclusion The loss of expression of P21 protein and the overexpression of survivin protein are associated with the malignant degree of gliomas.The P21 protein can inhibit the occurrence and development of glioma and the survivin protein can promote the development of glioma.

Key words: glioma, P21, survivin, immunohistochemistry

中图分类号:

R739.41

董志强, 李强, 贺振华, 潘亚文. P21和survivin蛋白在脑胶质瘤组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2015, 41(05): 1004-1007.

DONG Zhiqiang, LI Qiang, HE Zhenhua, PAN Yawen. Expressions of P21 and survivin proteins in glioma tissue and their clinical significances[J]. Journal of Jilin University Medicine Edition, 2015, 41(05): 1004-1007.

参考文献

[1] Dolecek TA,Propp JM,Stroup NE,et al. CBTRUS statistical report:primary brain and central nervous system tumors diagnosed in the United States in 2005-2009[J].Neuro Oncol,2012,14 (Suppl 5):v1-49.

[2] El-Deiry W,Tokino T,VelcuLescu V,et al.WAF1,a potential mediator of p53 tumor suppression[J].Cell,1993,75(4):817-825.

[3] Harper JW,Adami GR,Wei N,et al.The P21 cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases[J].Cell,1993,75(4):805-816.

[4] Kang TW,Choi SW,Yang SR,et al.Growth arrest and forced differentiation of human primary glioblastoma multiforme by a novel small molecule[J].Sci Rep,2014,3(4):5546.

[5] Harper JW,Elledge SJ,Keyomarsi K,et al.Inhibition of cyclin dependent kinases by P21[J].Mol Biol Cell,1995,6(4):387-400.

[6] Zhang H,Xiong Y,Beach D.Proliferating cell nuclear antigen and P21 are components of muLtiple cell cycle kinase complexes[J].Mol Biol Cell,1993,4(9):897-906.

[7] Mita AC,Mita MM,Nawrocki ST,et al.Survivin:key regulator of mitosis and apoptosis and novel target for cancer therapeutics[J].Clin Cancer Res,2008,14(16):5000-5005.

[8] Yamamoto H,Ngan CY,Monden M.Cancer cells survive with survivin[J].Cancer Sci,2008,99(9):1709-1714.

[9] 饶远权,王文宏,惠国桢.人脑胶质瘤组织中P21WAF1/CIP1表达及临床意义[J].中国肿瘤, 2006,15(12):865-866.

[10] Komiya T,Hosono Y,Hirashima T,et al.P21 expression as a predictor for favorable prognosis in squamous cell carcinoma of the lung[J].Clin Cancer Res,1997,3(10):1831-1835.

[11] Shi YZ,Hui AM,Takayama T,et al.Reduced P21WAF1/CIP1protein expression is predominantly related to altered p53 in hepatocelluLar carcinomas[J].Br J Cancer,2000,83(1):50-55.

[12] Takayasu T,Hama S,Yamasaki F,et al.p16 Gene transfer induces centrosome amplification and abnormal nucleation associated with survivin downregulation in glioma cells[J].Pathobiology,2015,82(1):1-8.

[13] Shen J,Liu J,Long Y,et al.Knockdown of survivin expression by siRNA enhances chemosensitivity of prostate cancer cells and attenuates its tumorigenicity[J].Acta Biochim Biophys Sin (Shanghai),2009,41(3):223-230.

[14] Hou JQ,He J,Wang XL,et al.Effect of small interfering RNA targeting survivin gene on biological behaviour of bladder cancer[J].Chin Med J (Engl),2006,119(20):1734-1739.

[15] 徐松柏,刘宝斌,赵红光,等. 靶向PTTG和survivin基因共干扰质粒对U251细胞的沉默效率[J].吉林大学学报:医学版,2012,38(1):37-41.

P21和survivin蛋白在脑胶质瘤组织中的表达及其临床意义

Expressions of P21 and survivin proteins in glioma tissue and their clinical significances

RichHTML

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	李红霞, 宫海叶, 曾萧, 张素素, 刘伯锋. TGF-β1和PLGF在不同类型妊娠滋养细胞疾病患者病变组织中的表达及其意义[J]. 吉林大学学报(医学版), 2018, 44(05): 1041-1046.
[2]	赵振理, 畅继武, 孙光, 胡少华. AAX14401蛋白在人膀胱癌组织中的表达及其意义[J]. 吉林大学学报(医学版), 2018, 44(05): 1052-1055.
[3]	王天月, 崔晓燕, 吴骁, 王丹. 磷酸化胞外信号调节激酶在小鼠海马发育过程中的表达及其意义[J]. 吉林大学学报(医学版), 2018, 44(05): 979-982.
[4]	阎慧, 娄石磊, 苗永迪, 岂蕊, 郭子硕, 邱悦, 孙聪. 大鼠结肠原代成纤维细胞的分离、培养和鉴定[J]. 吉林大学学报(医学版), 2018, 44(04): 849-852.
[5]	王淼, 母润红, 李明成, 李洪杰. RNAi沉默survivin和HIF-1α基因对胃癌BGC-823细胞体外增殖和凋亡的影响[J]. 吉林大学学报(医学版), 2018, 44(04): 753-758.
[6]	张啸环, 段明华, 闫玉礼, 潘新, 李海清, 周长龙, 赵天倚. 人羊膜成纤维细胞的分离和鉴定[J]. 吉林大学学报(医学版), 2018, 44(04): 845-848.
[7]	包幸福, 倪宇昕, 李莹, 郭小凯, 王玉琢, 方蛟, 胡敏. Wnt5a在小鼠牙骨质发育中的表达特点及其对成牙骨质细胞分化的影响[J]. 吉林大学学报(医学版), 2018, 44(03): 461-465.
[8]	马晓周, 刘麒麟, 尹唯凰, 王越, 于阳, 杨刚, 于子莹. CC趋化因子受体6在人唾液腺腺样囊性癌组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2018, 44(01): 147-150.
[9]	郑东春, 张天融, 刘洪泊, 陈怀基, 王琪, 许峰, 张月竹, 周丽婷, 叶琳. 染色体9p21遗传变异与中国汉族人群冠心病的关联性[J]. 吉林大学学报(医学版), 2018, 44(01): 142-146.
[10]	曹艳莎, 李婳, 陈明红, 刘宝琴, 王华芹, 李宁. 组织芯片技术检测BAG3蛋白在结肠癌组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2017, 43(06): 1177-1181.
[11]	高新雅, 张伟杰, 崔黎, 周学良, 马志俊, 吕转, 池艳艳, 王留兴. TM4SF1在乳腺癌组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2017, 43(06): 1186-1192.
[12]	杨娜, 刘国浩, 牟青春, 王学梅, 卞东林, 张震. 脑胶质瘤干细胞与转录因子NF-E2相关因子2关系的研究进展[J]. 吉林大学学报(医学版), 2017, 43(06): 1282-1286.
[13]	周世平, 徐红, 王琇, 李蓥. 内镜黏膜下剥离术治疗食管颗粒细胞瘤2例报告并文献复习[J]. 吉林大学学报(医学版), 2017, 43(05): 1034-1036.
[14]	历春, 付天悦, 杨宝学, 盖晓东. 尿素通道蛋白B在人膀胱癌组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2017, 43(04): 705-708.
[15]	石俊忠, 庄建彬, 张传山, 刘贤明, 孔大陆. β-tubulin Ⅲ在结肠腺癌组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2017, 43(04): 770-775.