当归挥发油对自发性高血压大鼠心肌组织中微小RNA差异表达谱的影响

doi:10.13481/j.1671-587x.20160407

吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (04): 671-675.doi: 10.13481/j.1671-587x.20160407

当归挥发油对自发性高血压大鼠心肌组织中微小RNA差异表达谱的影响

伊琳¹, 陈蓓蓓¹, 孙少伯², 杨陇³, 纪禄风¹, 石向慧¹

1. 甘肃中医药大学中西医结合学院内科学教研室, 甘肃兰州 730000;
2. 甘肃中医药大学基础医学院医用生物学教研室, 甘肃兰州 730000;
3. 甘肃省通渭县人民医院外科, 甘肃通渭743300

收稿日期:2015-11-20 发布日期:2016-07-20
通讯作者: 伊琳,副教授,硕士研究生导师(Tel:0931-8765463,E-mail:yxxxlyyy@163.com) E-mail:yxxxlyyy@163.com
作者简介:伊琳(1971-),女,陕西省三原县人,副教授,医学博士,主要从事心血管系统疾病方面的研究。
基金资助:
国家自然科学基金资助课题（81460669）；甘肃省科技厅第十批科技计划项目资助课题（1310RJZA084）

Effect of Angelica Sinensis Volatile Oil on miRNA differential expression profiling in myocardium tissue in spontaneously hypertensive rats

YI Lin¹, CHEN Beibei¹, SUN Shaobo², YANG Long³, JI Lufeng¹, SHI Xianghui¹

1. Department of Internal Medicine, College of Integrated Traditional and Western Medicine, Gansu University of Chinese Medicine, Lanzhou 730000, China;
2. Department of Medical Biology, College of Basic Medical Sciences, Gansu University of Chinese Medicine, Lanzhou 730000, China;
3. Department of Surgery, Tongwei People's Hospital, Gamsu Province, Tongwei 743300, China

Received:2015-11-20 Published:2016-07-20

摘要/Abstract

摘要：

目的：探讨当归挥发油对自发性高血压大鼠（SHRs）心肌组织中微小RNA（miRNA）表达谱的影响，阐明当归挥发油发挥作用的可能机制。方法：将SHRs分为模型组、卡托普利组和当归组，取同周龄的正常Wistar大鼠作为对照组，采用无创套尾法测定大鼠尾动脉收缩压，给药4周后，采用Affymetrix miRNA 4.0 芯片测定心肌组织中miRNA表达谱，采用京东基因与基因组百科全书（KEGG）富集法分析差异表达的miRNA靶基因。结果：给药前模型组大鼠收缩压明显高于对照组（P < 0.05），连续服药1~4周后，卡托普利组和当归组大鼠收缩压明显低于模型组（P < 0.05）。与模型组比较，当归组大鼠差异表达的miRNA29个，其中表达上调的miRNA13个（Ratio>2.0，P < 0.05），表达下调的miRNA16个（Ratio<0.5，P < 0.05）。KEGG富集分析，miR-19a、 let-7i和miR-181c与胰岛素信号通路有关联（P < 0.05），let-7i 、miR-181a和miR-455与血管内皮生长因子（VEGF）信号通路调控有关联（P < 0.05），miR-122、miR-181a、miR-200b、miR-181c、let-7i和miR-19a与细胞凋亡有关联（P < 0.05）。结论：当归挥发油可降低SHRs血压，对SHRs心肌组织中miRNA表达谱产生影响，可能通过调节胰岛素信号通路及VEGF信号通路相关miRNA发挥血压调节作用。

关键词: 当归挥发油, 大鼠, 近交SHR, 微小RNA表达谱

Abstract:

Objective: To explore the effect of Angelica Sinensis Volatile Oil on the mi RNA expression profiling in myocardium tissue of the spontaneously hypertensive rats (SHRs), and to clarify the possible mechanism of Angelica Sinensis Volatile Oil. Methods: All the SHRs were divided into Angelica group,model group and captopril group,and other Wistar rats with the same age were selected as control group. The non-invasive systolic blood pressure was detected; after 4 weeks of administration,the changes of miRNA expression profiling in myocardium tissue were measured by Affymetrix miRNA 4.0 Array. KEGG analysis was used to identify the target genes. Results: Compared with control group,the systolic blood pressure of the rats in model group was significantly decreased (P < 0.05) before treatment. 4 weeks after administration,compared with model group,the systolic blood pressure of the rats in Angelica group was significantly decreased(P < 0.05). Compared with model group,29 differential miRNAs of rats in Angelica group were found(P < 0.05),with 13 up-regulated miRNAs and 16 down-regulated miRNAs. The KEGG analysis results showed that miR-19a,let-7i, and miR-181c were related to insulin signaling pathways;let-7i,miR-181a,and miR-455 were related to VEGF signaling pathways; miR-122,miR-181a,miR-200b,miR-181c,let-7i,and miR-19a were related to apoptosis(P < 0.05). Conclusion: Angelica Sinensis Volatile Oil can decrease the blood pressure in SHRs and it can regulate the blood pressure by regulating the miRNA related to insulin signaling pathways and VEGF signaling pathways.

Key words: Angelica Sinensis Volatile Oil, rat,inbred SHR, miRNA expression profiling

中图分类号:

R544.1

伊琳, 陈蓓蓓, 孙少伯, 杨陇, 纪禄风, 石向慧. 当归挥发油对自发性高血压大鼠心肌组织中微小RNA差异表达谱的影响[J]. 吉林大学学报(医学版), 2016, 42(04): 671-675.

YI Lin, CHEN Beibei, SUN Shaobo, YANG Long, JI Lufeng, SHI Xianghui. Effect of Angelica Sinensis Volatile Oil on miRNA differential expression profiling in myocardium tissue in spontaneously hypertensive rats[J]. Journal of Jilin University Medicine Edition, 2016, 42(04): 671-675.

参考文献

[1] Schmiedel JM,Klemm SL,Zheng Y,et al. Gene expression. MicroRNA control of protein expression noise[J] . Science,2015,348(6230):128-132.

[2] Romaine SP,Tomaszewski M,Condorelli G,et al. MicroRNAs in cardiovascular disease:an introduction for clinicians[J]. Heart,2015,101(12):921-928.

[3] 程远,吕圭源,陈素红. 浅谈当归制剂降血压的临床应用[J] . 亚太传统医药,2011,7(4):138-139.

[4] 伊琳,赵昕,李屹. 当归对原发性高血压大鼠脑组织基因表达谱的影响[J]. 中国现代医学杂志,2014, 24(11):24-27.

[5] 伊琳,赵昕,李屹. 当归对自发性高血压大鼠脑组织 Tnfaip812、Ahsg 及 Tlr3 基因表达的影响[J]. 中国动脉硬化杂志,2013,21(10):891-893.

[6] 崔立建,刘瑞霞,王艳,等. ACE2-Ang (1-7) -Mas 轴的生物学功能及其下游信号通路[J]. 基础医学与临床,2014,34(12):1718-1722.

[7] Favre GA,Esnault VLM,van Obberghen E. Modulation of glucose metabolism by the rennin-angiotensin-aldoterone system[J]. Am J Physiol-Endocrinol Matabol,2015,308(6):E435-E449.

[8] Manrique C,Lastra G,Sowers JR. New insights into insulin action and resistance in the vasculature[J]. Ann N Y Acad Sci,2014,1311(1):138-150.

[9] Yang Z,Kaye DM. Mechanistic insights into the link between a polymorphism of the 3' UTR of the SLC7A1 gene and hypertension[J]. Hum Mutat,2009,30(3):328-333.

[10] Shibuya M . Vascular endothelial growth factor and its receptor system:physiological functions in angiogenesis and pathological roles in various diseases[J]. J Biochem,2013,153(1):13-19.

[11] Gerber HP ,McMurtrey A,Kowalski J,et al. Vascular endothelial growth factor regulates endothelial cell survival through the phosphatidylinositol 3'-kinase/Akt signal transduction pathway:requirement for Flk-1/KDR activation[J] . J Biol Chem, 1998,273(46):30336-30343.

[12] Kuehbacher A,Urbich C,Zeiher AM,et al. Role of Dicer and Drosha for endothelial microRNA expression and angiogenesis[J]. Circ Res, 2007,101(1):59-68.

[13] 钟南山,陆再英.内科学[M].北京:人民卫生出版社,2008:256-276.

[14] Moreau P ,Tea BS,Dam TV,et al. Altered balance between cell replication and apoptosis in hearts and kidneys of newborn SHR[J]. Hypertension,1997,30(3Pt2):720-724.

[15] Kobori H ,Ichihara A,Miyashita Y,et al. Local renin-angiotensin system contributes to hyperthyroidism-induced cardiac hypertrophy[J]. J Endocrinol,1999,160(1):43-47.

[16] Nadruz W. Myocardial remodeling in hypertension[J]. J Hum Hypertens,2015,29(1):1-6.

[17] Wang X ,Wang HX,Li YL,et al. MicroRNA Let-7i negatively regulates cardiac inflammation and fibrosis[J]. Hypertension,2015,66(4):776-785.

[18] Pan Z ,Sun X,Shan H,et al. MicroRNA-101 inhibited postinfarct cardiac fibrosis and improved left ventricular compliance via the FBJ osteosarcoma oncogene/transforming growth factor-β1 pathway[J]. Circulation,2012,126(7):840-850.

[19] Wei L,Yuan M,Zhou R,et al. MicroRNA-101 inhibits rat cardiac hypertrophy by rargeting Rab1a [J]. J Cardiovasc Pharmacol,2015,65(4):357-363.

[20] Yin K,Zhao L,Feng D,et al. Resveratrol Attenuated Low Ambient Temperature-Induced Myocardial Hypertrophy via Inhibiting Cardiomyocyte Apoptosis[J]. Cell Physiol Biochem,2015,35(6):2451-2462.

当归挥发油对自发性高血压大鼠心肌组织中微小RNA差异表达谱的影响

Effect of Angelica Sinensis Volatile Oil on miRNA differential expression profiling in myocardium tissue in spontaneously hypertensive rats

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