吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (04): 737-741.doi: 10.13481/j.1671-587x.20160420

• 基础研究 • 上一篇    下一篇

萱草花总黄酮含药血清对肝癌HepG2细胞增殖和凋亡的影响

沈楠1, 陈钱1, 齐玲2, 陈雪1, 黄成然1, 唐海如1, 安英1, 林峰1, 王艳春2   

  1. 1. 吉林医药学院实验中心, 吉林 吉林 132013;
    2. 吉林医药学院基础医学院病理学教研室, 吉林 吉林 132013
  • 收稿日期:2015-10-15 发布日期:2016-07-20
  • 通讯作者: 王艳春,教授,硕士研究生导师(Tel:0432-64560186,E-mail:wangyanchun1972@163.com) E-mail:wangyanchun1972@163.com
  • 作者简介:沈楠(1972-),女,吉林省吉林市人,副教授,医学硕士,主要从事药理学方面的研究。
  • 基金资助:

    吉林省教育厅科研基金资助课题(20130519);吉林省卫计委科技计划基金资助课题(2012Z060,2013ZC015);吉林省教育厅大学生创新创业训练项目资助课题(204029)

Effects of serum containing hemerocallis citrine baroni flavonids on proliferation and apoptosis of hepatoma HepG2 cells

SHEN Nan1, CHEN Qian1, QI Ling2, CHEN Xue1, HUANG Chengran1, TANG Hairu1, AN Ying1, LIN Feng1, WAN Yanchun2   

  1. 1. Experiment Center, Jilin Medical University, Jilin 132013, China;
    2. Department of Pathology, School of Basic Medical Sciences, Jilin Medical University, Jilin 132013, China
  • Received:2015-10-15 Published:2016-07-20

摘要:

目的:探讨萱草花总黄酮(HCBF)含药血清对人肝癌HepG2细胞增殖和凋亡的影响,阐明其作用机制。方法:HepG2细胞分为空白对照组及低(900 mg·kg-1)、中(1800 mg·kg-1)和高(2700 mg·kg-1)剂量HCBF组。采用MTT法检测HepG2细胞生长抑制率,倒置显微镜下观察HepG2细胞形态表现,AnnexinⅤ-PI双染和流式细胞术检测细胞凋亡率,酶联免疫吸附法检测细胞中凋亡蛋白Bax、bcl-2和Caspase-3表达水平。结果:与空白对照组比较,中和高剂量HCBF组HepG2细胞生长抑制率明显升高(P < 0.05或P < 0.01)。倒置显微镜下观察,HCBF组HepG2细胞体积缩小,形状不规则,细胞核呈浓缩和破碎状态。与空白对照组比较,中和高剂量HCBF组HepG2细胞凋亡率升高(P < 0.05或P < 0.01),bcl-2蛋白表达水平明显降低(P < 0.05或P < 0.01),Bax蛋白表达水平明显升高(P < 0.05或P < 0.01);高剂量HCBF组Caspase-3蛋白表达水平明显升高(P < 0.05)。结论:HCBF含药血清可以诱导HepG2细胞凋亡,其机制可能与线粒体途经有关联。

关键词: 萱草花总黄酮, 血清药理学, HepG2细胞, 细胞增殖, 细胞凋亡

Abstract:

Objective: To explore the effects of serum containing hemerocallis citrine baroni flavonids (HCBF) on the proliferation and apoptosis of human hepatoma HepG2 cells,and to clarify their mechanisms. Methods: The HepG2 cells were divided into blank control group,low (900 mg·kg-1),middle (1 800 mg·kg-1) and high (2 700 mg·kg-1) doses of serum containing HCBF groups. The inhibitory rates of growth of HepG2 cells were measured with MTT assay; inverted microscope was used to observe the morphologic changes; Annexin Ⅴ-PI double staining and flow cytometry were used to detect apoptotic rates of HepG2 cells;enzyme linked immunosorbent assay was used to detect the expression levels of Bax,bcl-2, and Caspase-3. Results: Compared with blank control group,the inhibitory rates of growth of HepG2 cells in middle and high doses of HCBF groups were increased (P < 0.05 or P < 0.01).The cells were shrank with irregular shape,and the nucleus was condensed and broken in HCBF group under inverted microscope.Compared with blank control group,the apoptotic rates of HepG2 cells in middle and high doses of HCBF groups were increased (P < 0.05 or P < 0.01). Compared with blank control group,the expression levels of bcl-2 protein in middle and high doses of HCBF groups were decreased(P < 0.05 or P < 0.01),and the expression levels of Bax protein were significantly increased (P < 0.05 or P< 0.01),and the expression level of Caspase-3 protein in high dose of HCBF group was significantly increased(P < 0.05). Conclusion: The serum containing HCBF could induce the apoptosis of HepG2 cells,and its mechanism may be related to mitochondrial pathway.

Key words: hemerocallis citrine baroni flavonids, serum pharmacology, HepG2 cells, cell proliferation, apoptosis

中图分类号: 

  • R7285.5