伏立康唑对体外培养棘阿米巴增殖和形态表现的影响

doi:10.13481/j.1671-587x.20170311

吉林大学学报(医学版) ›› 2017, Vol. 43 ›› Issue (03): 518-521.doi: 10.13481/j.1671-587x.20170311

伏立康唑对体外培养棘阿米巴增殖和形态表现的影响

王月华¹, 鞠晓红¹, 钟秀宏², 李强¹, 孙艳美¹, 姜晓明¹

1. 吉林医药学院检验学院病原技术教研室, 吉林吉林 132013;
2. 吉林医药学院基础医学院病理学教研室, 吉林吉林 132013

收稿日期:2016-09-05 出版日期:2017-05-28 发布日期:2017-06-01
通讯作者: 王月华,副教授(Tel:0432-64560539,E-mail:yuehua26@163.com) E-mail:yuehua26@163.com
作者简介:王月华(1979-),女,吉林省吉林市人,副教授,医学硕士,主要从事寄生虫感染免疫方面的研究。
基金资助:
吉林省教育厅科研基金资助课题（2014373）

Effects of voriconazole on proliferation and morphology of Acanthamoeba cultivated in vitro

WANG Yuehua¹, JU Xiaohong¹, ZHONG Xiuhong², LI Qiang¹, SUN Yanmei¹, JIANG Xiaoming¹

1. Department of Pathogen Technology, School of Laboratory, Jilin Medical University, Jilin 132013, China;
2. Department of Pathology, School of Basic Medical Sciences, Jilin Medical University, Jilin 132013, China

Received:2016-09-05 Online:2017-05-28 Published:2017-06-01

摘要/Abstract

摘要： 目的：探讨伏立康唑对体外培养棘阿米巴的增殖和形态表现的影响，阐明伏立康唑对棘阿米巴滋养体和包囊的抑制和杀伤作用。方法：选取对数生长期的棘阿米巴原虫分为对照组和实验组（2.5 和25.0 mg·L^-1），分别于药物作用后24、48、72和96 h收集各组虫体，计数虫体数量并绘制增殖曲线；光镜下观察棘阿米巴的形态表现、活力和贴壁情况；电镜下观察其超微结构。结果：与对照组比较，实验组棘阿米巴虫体数量减少（P<0.01）；光镜下实验组棘阿米巴形态变化明显，由不规则带棘状伪足的滋养体转化为圆形包囊，出现大量细胞碎片；电镜下实验组棘阿米巴的超微结构出现不同程度的破坏、坏死。结论：一定浓度伏立康唑能够有效抑制体外培养棘阿米巴的增殖并改变其形态甚至超微结构，对滋养体和包囊均具有明显杀伤作用。

关键词: 棘阿米巴, 伏立康唑, 形态学, 细胞增殖

Abstract: Objective: To explore the effects of voriconazole on the proliferation and morphology of Acanthamoeba cultivated in vitro,and to clarify the killing effects of voriconazole against the trophozoites and cysts of Acanthamoeba. Methods: The Acanthamoeba polyphaga at logarithmic phase were selected and divided into control group and experiment groups(2.5 and 25.0 mg·L^-1).The Acanthamoeba in each group was collected at 24,48,72, and 96 h after drug administration,respectively.Then the concentrations of Acanthamoeba were calculated and the proliferation curves were drawn;inverted microscope was used to observe the morphology,activity and adherence of Acanthamoeba;the ultrastructures of Acanthamoeba were observed under electron microscope. Results: Compared with control group,the numbers of Acanthamoeba polyphaga in experiment groups were significantly decreased(P<0.01).The morphology of Acanthamoeba changed significantly under inverted microscope,and the shape of Acanthamoeba transformed from the trophozoites with irregular spiny filopodia to circular cysts.Even a large number of cell debris was observed.Different degrees of damage and even necrosis of Acanthamoeba in experiment groups were found under electron microscope. Conclusion: Certain concentration of voriconazole can effectively inhibit the proliferation of Acanthamoeba and change the morphology and ultrastructure and kill the trophozoites and cysts of Acanthamoeba cultivated in vitro.

Key words: morphology, voriconazole, Acanthamoeba, cell proliferation

中图分类号:

Q939

王月华, 鞠晓红, 钟秀宏, 李强, 孙艳美, 姜晓明. 伏立康唑对体外培养棘阿米巴增殖和形态表现的影响[J]. 吉林大学学报(医学版), 2017, 43(03): 518-521.

WANG Yuehua, JU Xiaohong, ZHONG Xiuhong, LI Qiang, SUN Yanmei, JIANG Xiaoming. Effects of voriconazole on proliferation and morphology of Acanthamoeba cultivated in vitro[J]. Journal of Jilin University Medicine Edition, 2017, 43(03): 518-521.

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伏立康唑对体外培养棘阿米巴增殖和形态表现的影响

Effects of voriconazole on proliferation and morphology of Acanthamoeba cultivated in vitro

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