关键词: 纯化, 山葡萄籽, 多酚, 大孔树脂

Abstract: Objective:To explore the purification technology parameters for polyphenols from vitis amurensis seed extract with AB-8 macroporous resin and to establish the optimum purification process conditions, and to provide technical reference for further development and utilization of vitis amurensis seeds. Methods:The vitis amurensis seeds were regarded as raw material, the concentration of vitis amurensis seed polyphenols was detected,and the adsorption rate and desorption rate of polyphenols were calculated. The AB-8 macroporous resin was used to purify the polyphenols from vitis amurensis seed extract,and the purification technology parameters of vitis amurensis seed polyphenols were optimized by absorption-desorption experiment.The concentrations of polyphenol from vitis amurensis seed extract were compared between before and after purification,and the effect of the purification process was verified. Results:The AB-8 macroporous resin had good absorption and desorption properties to crude the extract of vitis amurensis seed polyphenols,and the adsorption rate and desorption rate were 90.48% and 71.42%.The optimum adsorption conditions and desorption conditions were as follows:the sample concentration was 10 g·L^-1, the sample flow rate was 3 mL·min^-1, the eluting concentration was 60%,the elution velocity was 2 mL·min^-1,and the eluting volume was 2-fold column volume. Under this condition,the concentration of polyphenols from vitis amurensis seed extract after purification was increased from 35.02% to 88.8%. Conclusion:AB-8 macroporous resin has better purification effect,and it is worthy of popularization and application.

Key words: polyphenols, macroporous resin, purification, vitis amurensis seeds