白藜芦醇对大鼠视网膜缺血再灌注损伤后视网膜组织中Caspase-3和Bcl-2表达的影响

doi:10.13481/j.1671-587x.20180221

摘要/Abstract

摘要： 目的：研究白藜芦醇对大鼠视网膜缺血再灌注损伤（RIRI）后视网膜组织中Caspase-3和Bcl-2表达的影响，探讨白藜芦醇对RIRI的治疗作用及其机制。方法：选取SD大鼠90只，随机分为假手术组、模型组和治疗组，每组30只，通过对前房加压建立大鼠RIRI模型，模型组和治疗组大鼠缺血再灌注1、6、12、24和48 h，治疗组大鼠用微量注射器于大鼠玻璃体腔内注射0.5 nmol·L^-1的白藜芦醇5 μL。采用倒置显微镜观察视网膜组织结构，采用免疫组织化学法和Western blotting法检测各组大鼠视网膜组织中Caspase-3和Bcl-2的阳性细胞数和蛋白表达水平。结果：模型组大鼠造模后视网膜组织水肿，可见神经节细胞空泡样变性，细胞层排列呈现疏松状，视网膜神经节细胞数目大量减少，边界模糊，神经纤维层明显变薄；治疗组大鼠视网膜组织结构、损伤程度及神经节细胞变性程度均轻于模型组。免疫组织化学检测，与模型组比较，治疗组大鼠视网膜组织中Caspase-3和Bcl-2阳性细胞数明显升高（P<0.05）。Western blotting法检测，与模型组比较，治疗组大鼠各时间点视网膜组织中Bcl-2蛋白表达水平升高，24和48 h时差异有统计学意义（P<0.05）；治疗组各时间点大鼠视网膜组织中Caspase-3蛋白表达水平均明显降低（P<0.05）。结论：白藜芦醇对RIRI大鼠的视网膜神经节细胞结构有改善作用，其作用机制可能与降低视网膜组织中Caspase-3表达水平及升高视网膜组织中Bcl-2表达水平有关。

关键词: 白藜芦醇, 视网膜缺血再灌注损伤, Caspase-3, Bcl-2

Abstract: Objective:To study the effects of resveratrol on the expressions of Caspase-3 and Bcl-2 in the retina tissue of the rats with retinal ischemia-reperfusion injury (RIRI),and to investigate the therapeutic effect of resveratrol on the RIRI and its mechanism. Methods: A total of 90 SD rats were randomly divided into sham operation group,model group and treatment group,30 rats in each group.The RIRI models were established by pressing the anterior chamber of the rats.The rats in model and treatment groups received ischemia-reperfusion for 1,6,12,24,and 48h;the rats in treatment group were treated with micro-syringe intravitreal injection of 0.5 nmol·L^-1 of resveratrol 5 μL.The retinal tissue structure was observed by inverted microscope.The expression levels of Caspase-3 and Bcl-2 in retina tissue were detected by immunohistochemistry and Western blotting methods. Results: The retinal tissue edema of the rats in model group was found with vacuolar degeneration of the ganglion cells,and the arrangement of the cell layer was loose;the number of retinal ganglion cells was decreased,the boundary was blurred,and the nerve fiber layer thinned obviously.The degree of retinal tissue structure,the degree of injury and the degeneration of ganglion cells in treatment group were lighter than those in model group.The results of immunohistochemistry showed that the expressions of Caspase-3 and Bcl-2 in the retina tissue of the rats in treatment group were significantly increased compared with model group.The results of Western blotting method showed that the expression levels of Bcl-2 protein in the retina tissue of the rats in treatment group at different time points were increased compared with model group;and there were significant differences at 24 and 48 h (P<0.05);the expression levels of Caspase-3 protein in treatment group at different time points were lower than those in model group (P<0.05). Conclusion: Resveratrol can improve the retinal tissue structure of the RIRI rats,and its mechanism may be related to decreasing the expression level of Caspase-3 and increasing the expression level of Bcl-2 in the retinal tissue.

Key words: Bcl-2, resveratrol, retinal ischemia-reperfusion injury, Caspase-3

中图分类号:

R285.5

苏畅, 周海生, 郭淑玲, 苏锐锋, 付笑笑, 谭小波, 李伟. 白藜芦醇对大鼠视网膜缺血再灌注损伤后视网膜组织中Caspase-3和Bcl-2表达的影响[J]. 吉林大学学报(医学版), 2018, 44(02): 321-326.

SU Chang, ZHOU Haisheng, GUO Shuling, SU Ruifeng, FU Xiaoxiao, TAN Xiaobo, LI Wei. Effects of resveratrol on expressions of Caspase-3 and Bcl-2 in retina tissue of rats with retinal ischemia-reperfusion injury[J]. Journal of Jilin University Medicine Edition, 2018, 44(02): 321-326.

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