吉林大学学报(医学版) ›› 2018, Vol. 44 ›› Issue (03): 532-536.doi: 10.13481/j.1671-587x.20180314

• 基础研究 • 上一篇    下一篇

褪黑素对人非小细胞肺癌H1299细胞辐射敏感性的影响

杨文延, 刘强, 孙志娟, 杜利清, 徐畅, 王彦, 柳杨, 王芹   

  1. 中国医学科学院北京协和医学院放射医学研究所天津市放射医学与分子核医学重点实验室, 天津 300192
  • 收稿日期:2017-12-29 出版日期:2018-05-28 发布日期:2018-05-31
  • 通讯作者: 王芹,副研究员,硕士研究生导师(Tel:022-85683047,E-mail:wangqin@irm-cams.ac.cn) E-mail:wangqin@irm-cams.ac.cn
  • 作者简介:杨文延(1992-),女,河北省邢台市人,在读医学硕士,从事放射生物学方面的研究。
  • 基金资助:
    天津市科委自然科学基金资助课题(09JCYBJC09300);北京协和医学院教学改革项目资助课题(院1444)

Effect of melatonin on radiosensitivity of non-small cell lung cancer H1299 cells

YANG Wenyan, LIU Qiang, SUN Zhijuan, DU Liqing, XU Chang, WANG Yan, LIU Yang, WANG Qin   

  1. Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192, China
  • Received:2017-12-29 Online:2018-05-28 Published:2018-05-31

摘要: 目的:检测褪黑素(MLT)联合放疗对人非小细胞肺癌(NSCLC) H1299细胞增殖和凋亡的影响,阐明MLT在调节H1299细胞辐射敏感性中的作用。方法:将H1299细胞分为对照组、MLT组、照射组及照射+MLT组,对照组H1299细胞不作任何处理,MLT组H1299细胞给予不同浓度(100和500 μmol·L-1) MLT,照射组H1299细胞采用137Cs γ射线进行一次性6 Gy照射,照射+MLT组H1299细胞给予100和500μmol·L-1 MLT加6 Gy137Cs γ射线。克隆形成实验检测各组细胞克隆形成数,流式细胞术检测照射后24和48 h各组细胞凋亡率,蛋白免疫印迹法检测各组细胞中核转录因子κB(NF-κB)蛋白的磷酸化水平。结果:克隆形成实验,与照射组比较,照射+MLT组H1299细胞克隆形成数明显减少(t=7.234,P<0.01)。流式细胞术,照射后24 h,与对照组比较,照射组和MLT组H1299细胞凋亡率明显升高(t=5.020,t=13.525,P<0.01),与对照组比较,照射+MLT组H1299细胞凋亡率升高(t=12.884,P<0.01);照射后48 h,照射+MLT组H1299细胞凋亡率与照射组比较差异无统计学意义(t=0.394,P>0.05)。蛋白免疫印迹法,照射+MLT组H1299细胞中NF-κB蛋白磷酸化水平低于照射组。结论:MLT联合放疗对人NSCLC H1299细胞有明显的抑瘤效应,提高了NSCLCH1299细胞的辐射敏感性。

关键词: 褪黑素, 细胞增殖, 细胞凋亡, 癌, 非小细胞肺, 核转录因子κB, 辐射敏感性

Abstract: Objective: To detect the effects of melatonin (MLT) combined with radiotherapy on the proliferation and apoptosis of non-small cell lung cancer (NSCLC) H1299 cells,and to explore the role of MLT in regulating the radiosensitivity of H1299 cells. Methods: The H1299 cells were divided into control group,MLT group,irradiation(IR) group and IR+MLT group. The H1299 cells in control group didn't receive any treatment, the H1299 cells in MLT group were treated with different concentrations of MLT (100 and 500 μmol·L-1),the H1299 cells in IR group were exposed to 6 Gy 137Cs γ-ray,and the H1299 cells in IR+MLT group were treated with different concentrations of MLT (100 and 500 μmol·L-1) and exposed to 6 Gy 137Cs γ-ray. Colony formation assay was used to detect the clone numbers of H1299 cells in various groups; Flow cytometry was applied to detect the apoptotic rates of H1299 cells at 24 and 48 h after irradiation; Western blotting method was performed to detect the phosphorylation levels of nuclear factor kappa B (NF-κB) protein in the H1299 cells in various groups. Results: The colony formation assay results showed that compared with IR group,the clone number of H1299 cells in IR+MLT group was significantly decreased(t=7.234,P<0.01).The flow cytometry results showed that the apoptotic rates of H1299 cells in IR group and MLT group at 24 h after irradiation were higher than that in control group (t=5.020,t=13.525,P<0.01); the apoptotic rate of H1299 cells in IR+MLT group at 24 h after irradiation was higher than that in IR group (t=12.884,P<0.01).There was no significant difference in the apoptotic rate of the H1299 cells between IR+MLT group and IR group at 48 h after irradiation(t=0.394,P>0.05).The Western blotting results showed that the phosphorylation level of NF-κB protein in the H1299 cells in IR+MLT group was lower than that in IR group. Conclusion: MLT combined with radiotherapy has obviously inhibitory effect on the human H1299 cells and can increase the radiosensitivity of H1299 cells.

Key words: melatonin, nuclear factor kappa B, cancer,non-small cell lung, proliferation, apoptosis, radiosensitivity

中图分类号: 

  • Q691