ITGA1基因多态性与幽门螺杆菌感染的关联性分析

doi:10.13481/j.1671-587x.20180628

吉林大学学报(医学版) ›› 2018, Vol. 44 ›› Issue (06): 1275-1279.doi: 10.13481/j.1671-587x.20180628

ITGA1基因多态性与幽门螺杆菌感染的关联性分析

马立聪^1,2, 高芳³, 董文杰¹, 田旭阳², 党彤², 贾彦彬^1,2

1. 包头医学院基础医学与法医学院, 内蒙古包头 014060;
2. 包头医学院第二附属医院内蒙古消化病研究所, 内蒙古包头 014030;
3. 包头医学院医学技术学院, 内蒙古包头 014060

收稿日期:2018-03-21 出版日期:2018-11-28 发布日期:2018-11-28
通讯作者: 贾彦彬,教授,硕士研究生导师(Tel:0472-7167832,E-mail:jyb690318@hotmail.com) E-mail:jyb690318@hotmail.com
作者简介:马立聪(1990-),男,内蒙古自治区包头市人,检验师,在读医学硕士,主要从事复杂疾病易感基因方面的研究。
基金资助:
内蒙古自治区高等学校科研项目资助课题（NJZY204）；包头医学院科研基金资助课题（BYJJ-DF-201603）

Analysis on association between polymorphism of ITGA1 gene and Helicobacter pylori infection

MA Licong^1,2, GAO Fang³, DONG Wenjie¹, TIAN Xuyang², DANG Tong², JIA Yanbin^1,2

1. School of Basic Medicine and Forensic Medicine, Baotou Medical College, Baotou 014060, China;
2. Inner Mongolia Institute of Digestive Diseases, Second Affiliated Hospital, Baotou Medical College, Baotou 014030, China;
3. School of Medical Technology, Baotou Medical College, Baotou 014060, China

Received:2018-03-21 Online:2018-11-28 Published:2018-11-28

摘要/Abstract

摘要： 目的：探讨整联蛋白α亚基1（ITGA1）基因单核苷酸多态性（SNP）与幽门螺杆菌感染的关联性，为幽门螺杆菌感染相关疾病的防治提供依据。方法：选择体检者281人的血液样本，采用ELISA法确定体验者血液中幽门螺杆菌的感染状况，根据抗体滴度将样本分为幽门螺杆菌阴性组（n=159）和幽门螺杆菌阳性组（n=122）。采用TaqMan法对ITGA1基因的rs1862610、rs2432143和rs2447867位点进行基因分型，采用Haploview 4.0软件构建单体型，采用非条件性Logistic回归检测各等位基因、基因型的SNPs和单体型与幽门螺杆菌感染的关系。结果：ITGA1基因rs1862610和rs2432143位点的SNPs与幽门螺杆菌感染无关联（P>0.05）；ITGA1基因rs2447867位点的SNP与幽门螺杆菌感染有关联（P<0.05），携带基因型CT可降低幽门螺杆菌感染风险（CT vs CC：OR=0.52，95% CI：0.31~0.86）。ITGA1基因rs1862610和rs2432143位点SNPs之间存在强连锁不平衡（D'=1），构成单体型块，各单体型与幽门螺杆菌感染无关联（P>0.05）。结论：ITGA1基因rs2447867位点的SNP与幽门螺杆菌感染有关联，携带基因型CT可降低幽门螺杆菌感染风险。

关键词: 幽门螺杆菌, 胃肿瘤, 整联蛋白, 基因多态性, 单核苷酸多态性

Abstract: Objective: To investigate the association between single nucleotide polymorphism(SNP) of integrin α-subunit 1 (ITGA1) gene and Helicobacter pylori(H. pylori) infection, and to provide the basis for the prevention and treatment of H. pylori infection-related diseases.Methods: The blood samples of 281 examinees were detected. ELISA method was used to confirm the status of H. pylori infection in the blood of the examinees.The samples were divided into H. pylori negative group(n=159) and H. pylori positive group(n=122)according to the antibody titers. The genotypes of rs1862610, rs2432143, and rs2447867 sites in ITGA1 gene were detected by TaqMan method.The haplotype was constructed by Haploview software 4.0 and unconditional Logistic regression was used to evaluate the associations between the SNPs of alleles, genotypes and haplotypes and H. pylori infection.Results: The SNPs of rs1862610 and rs2432143 sites in ITGA1 gene were not associated with H. pylori infection(P>0.05). The SNPs of rs2447867 site in ITGA1 gene was significantly associated with H. pylori infection(P<0.05). Carrying CT genotype decreased the risk of H. pylori infection (CT vs CC:OR=0.52, 95% CI:0.31-0.86). The SNPs between rs1862610 and rs2432143 sites in ITGA1 gene had a strong linkage disequilibrium (D'=1) and formed a block. None of the haplotypes was associated with H. pylori infection(P>0.05).Conclusion: The SNP of rs2447867 site in ITGA1 gene is associated with H. pylori infection,and carrying CT genotype can decrease the risk of H. pylori infection.

Key words: Helicobacter pylori, stomach tumor, integrin, gene polymorphism, single nucleotide polymorphism

中图分类号:

R378.21

马立聪, 高芳, 董文杰, 田旭阳, 党彤, 贾彦彬. ITGA1基因多态性与幽门螺杆菌感染的关联性分析[J]. 吉林大学学报(医学版), 2018, 44(06): 1275-1279.

MA Licong, GAO Fang, DONG Wenjie, TIAN Xuyang, DANG Tong, JIA Yanbin. Analysis on association between polymorphism of ITGA1 gene and Helicobacter pylori infection[J]. Journal of Jilin University(Medicine Edition), 2018, 44(06): 1275-1279.

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ITGA1基因多态性与幽门螺杆菌感染的关联性分析

Analysis on association between polymorphism of ITGA1 gene and Helicobacter pylori infection

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