婴幼期大自然声音组合干预对大鼠成年后焦虑样行为的影响

doi:10.13481/j.1671-587X.20210409

摘要/Abstract

摘要： 目的

通过测试子代雄性SD大鼠成年后的焦虑样行为，阐明婴幼期声音干预对大鼠成年后焦虑样行为的影响。

方法

6只孕14~15 d的SD大鼠随机分为正常噪声环境对照组（NN组）、莫扎特D大调双钢琴奏鸣曲K448组（MK组）和大自然声音组合组（NS组）。NN组大鼠不给予任何特殊的声音刺激；MK组大鼠每天给予莫扎特D大调双钢琴奏鸣曲K448声音刺激2 h，音量为50~70 dB，持续30 d；NS组大鼠每天给予大自然声音组合刺激2 h，音量为65~75 dB，持续30 d。刺激结束后5周，选取体质量相近的NN组11只、MK组8只和NS组11只雄性子代大鼠进行旷场实验、明暗箱实验和高架十字迷宫实验。旷场实验检测大鼠总运动路程、站立次数、进入中央区域次数和进入中央区域的时间，明暗箱实验检测大鼠在明箱停留时间百分率、穿梭次数和总运动路程，高架十字迷宫实验检测大鼠在开臂的时间百分率、进入次数和运动路程。

结果

旷场实验中3组大鼠的总运动路程、站立次数、进入中央区域次数和进入中央区域的时间比较差异均无统计学意义（P>0.05）。明暗箱实验，与NN组比较，NS组大鼠在明箱停留时间百分率明显升高（P<0.05）；与NN组和MK组比较，NS组大鼠在明暗箱之间的穿梭次数和总运动路程明显增加（P<0.05或P<0.01）。高架十字迷宫实验，与NN组比较， NS组大鼠在开臂的时间百分率明显升高（P<0.05）。

结论

婴幼期大自然声音的干预可减少雄性SD大鼠成年后的焦虑样行为。

关键词: 自然环境, 自然界声音, 音乐疗法, 焦虑, 大鼠,SD

Abstract: Objective

To clarify the effect of combined natural sound intervention during infancy on the anxiety-like behaviors in the adult rats by testing the offspring male SD rats’ anxiety-like behaviors.

Methods

Six SD rats with 14－15 d of gestation were randomly divided into standard noise control group （NN group），Mozart K448 group （MK group） and combination of natural sound group （NS group）. The rats in NN group were not given any special sound stimulation，the rats in MK group were given 2 h of MK sound stimulation at a volume of 50－70 dB per day for 30 d，and the rats in NS group were given a combination of natural sounds at a volume of 65－75 dB for 2 h a day for 30 d. Five weeks after the end of stimulation， 11 male rats with the similar body weight in NN group and in NS group， and 8 rats in MK group were selected for open field experiment，light-dark transition test and elevated plus-maze test.The open field experiment was used to analyze the total movement distance， rearing times， the number of entering the central area， and the time of entering the central area. The percentage of time staying in light box and the number of transition between light and dark boxes and the total movement distance of rats were analyzed in the light-dark transition test. The elevated plus-maze test was used to analyze the percentage of time in open arms， the number of open arms entries and the distance of open arms.

Results

In the open field experiment， there were no statistically significant differences among three groups in the total movement distance， rearing times， the time of entering the central area， and the number of entering the central area （P>0.05）. The results of light-dark transition test showed that the time in the light box of the rats in NS group was significantly longer than that in NN group （P<0.05）； the number of transition between light and dark boxes of the rats in NS group was also significantly increased （P<0.05 or P<0.01）. The results of elevated plus-maze test also showed that the percentage of time in open-arms of the rats in NS group was significantly longer than that in NN group （P<0.05）.

Conclusion

Intervention of natural sounds during infancy can reduce the anxiety-like behaviors in the mature male SD rats.

Key words: natural environment, natural sounds, music trerapy, anxiety, rats，SD

中图分类号:

R749.059

王倩,庄似玥,吴贵龙,李胜天. 婴幼期大自然声音组合干预对大鼠成年后焦虑样行为的影响[J]. 吉林大学学报(医学版), 2021, 47(4): 882-887.

Qian WANG,Siyue ZHUANG,Guilong WU,Shengtian LI. Effect of combination of natural sounds during infancy on anxiety-like behaviors in adult rats[J]. Journal of Jilin University(Medicine Edition), 2021, 47(4): 882-887.

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Group	n	Total distance(l/m)	Number of rearing	Number of entering central area	Time of entering central area(t/s)
NN	11	13.52±0.90	39.27±3.88	14.18±2.61	24.34±4.12
MK	8	12.11±0.93	34.38±3.49	16.63±3.72	25.41±5.60
NS	11	11.50±1.40	32.63±4.98	8.82±2.12	15.75±4.70

Group	n	Percentage of time in light box(η/%)	Number of transition	Total distance(l/m)
NN	11	32.28±3.46	15.73±1.41	7.35±1.29
MK	8	34.38±1.91	17.88±1.73	6.43±0.33
NS	11	41.38±2.53^*	26.27±2.17^**△	9.16±0.99^*△

Group	n	Percentage of time in open arms(η/%)	Number of open arms entries	Distance of open arms(l/m)
NN	11	11.99±3.94	4.73±1.07	0.83±0.25
MK	8	23.52±6.85	6.28±1.33	1.28±0.39
NS	11	25.06±2.45^*	4.82±0.46	1.24±0.17

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