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• 基础研究 • 上一篇    下一篇

大鼠附睾9型水通道表达

杨 柯1,赵 丹1,杨宝学2,王蔚东3,赵雪俭1*,徐桂香4   

  1. 1. 吉林大学基础医学院病理生理学教研室,吉林 长春130021;2. 美国加利福尼亚大学旧金山医学院心血管研究所医学生理学研究室,加利福尼亚 旧金山CA94122;3. 丹麦Aarthus大学解剖学研究所细胞生物学研究室;4. 吉林
  • 收稿日期:2003-04-28 修回日期:1900-01-01 出版日期:2004-07-28 发布日期:2004-07-28

Expressions of aquaporin 9 in capita andcaudae epididymides of adult rats

YANG Ke1, ZHAO Dan1, YANG Bao-xue2, WANG Wei-dong3, ZHAO Xue-jian1, XU Gui-xiang4   

  1. 1. Department of Pathophysiology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China;2. Department of Medicine and Physiology, Cardiovascular Research Institute, Medical Collegeof San Francisco, University of California, San Francisco CA94122,USA;3. Department of Cell Biology, Institute of Anatomy, University of Aarhus,Denmark;4. Laboratory of IVF, Institute of Family Planning,Jilin Province, Changchun 130021, China
  • Received:2003-04-28 Revised:1900-01-01 Online:2004-07-28 Published:2004-07-28

摘要: 目的:观察大鼠附睾9型水通道(aquaporin 9,AQP9)的表达。方法:取12周龄大鼠的附睾头部和尾部提取总RNA和总蛋白进行AQP9 Northern blotting分析和Western blotting分析。结果:12周龄大鼠附睾存在AQP9的表达,且附睾头部表达强于尾部。结论:12周龄大鼠附睾头部AQP9表达强于尾部,反映附睾不同部位对水的重吸收功能的差异。

关键词: 代谢, 附睾, 代谢, 免疫组织化学, 方法, 疾病模型, 动物

Abstract: Objective To examine the expressions of aquaporin 9(AQP9) in various segments epididymides of adult rats. Methods The capita and caudae epididymides of 12 week rats were removed for total RNA extraction and total protein purification. Northern blotting analysis was performed with AQP9 cDNA probe, and Western blotting was performed with polyclonal anti-AQP9 antibody. Results The expressions of AQP9 were confirmed in rat epididymides and the AQP9 expression in capita epididymides was higher than that in caudae epididymides. Conclusion AQP9 expression is found in rat epididymides. The expression levels of AQP9 in rat capita and caudae epididymides show the reabsorption capacity in different segments of epididymides.

Key words: metabolism, epididymis, metabolism, immunohistochemistry, methods, disease models, animal

中图分类号: 

  • R339.21