关键词: 抗体, 单克隆, 分离和提纯, 酶联免疫吸附测定

Abstract: Objective To prepare anti-osteosarcoma monoclonal antibodies (McAb) and study its characteristics. Methods Splenocytes of BALB/c mice immunized with osteosarcoma cell line OS-732 were extracted and these cells were fused with murine myeloma cell line NS-1. Enzyme-linked immunosorbent assay (ELISA) and complement-dependent cytotoxic assay were used to screen and three hybriddoma cell lines capable of screting antiosteosarcoma monoclonal antibodies were acquired. The tissues of osteosarcoma, chondrosarcoma, giant cell tumor of bone,leiomyosarcoma, synoviosarcoma, nonossifying fibroma,osteochondroma, normal tissues of muscle and bone were made into frozen slides and the indirect immunofluorescence test were applied to test these specimens. Results All the three lines showed positive results in the osteosarcoma slides and two of them showed weakly positive results in the chondrosarcoma. The three McAb lines combined with different antigenic determinant epitope of osteosarcomous correlative antigens, respectively. Conclusion McAb possesses higher sensitivity and specificity.

Key words: antibodies, monoclonal, isolation & purification, enzyme-linked immunosorbent assay