关键词: 模型, 动物, 受体, 肾上腺素能β_2<, sub>

Abstract: Abstract:Objective To explore the expression of beta 2 adrenergic receptors(β₂-AR) in bone marrow cells and its significance and provide a scientific basis for the theory of hematopoietic regulation by nerve factors. Methods After　 bone marrow mononuclear cells were isolated from normal mouse bone marrow by the Ficoll density gradient centrifugation，the expressions of β₂-AR were studied by immunohistochemistry in bone marrow mononuclear cells,cultured bone marrow stromal cells,GM-CFUs,paraffin sections and smears of bone marrow.Mouse models of immune-mediated aplastic anemia(AA) were made by lymphocyte infusion.Mice were divided into 3 groups including animal model group,β₂-AR antagonist (ICI 118551) group,and control group,and the expression of β₂-AR in bone marrow mononuclear cells at the 8 th day was dete cted by Western blotting.Results The　 positive expression of β₂-AR in cytoplasm or membrane of normal mouse bone marrow cells showed different brown by immunohistochemical staining.There were intensive positive expressions of β₂-AR in mononuclear cells(48%) and bone marrow stromal cells (68%),while progenitor cells only showed middle-positive(55%)or weak positive(45%),and other hematopoietic accessory cells were positive in varying degrees.The relative values of β₂-AR of bone marrow mononuclear cells in 3 groups were 1.03±0.31,1.72±0.29 and 1.41±0.35,respectively;the expression of β₂-AR in animal model group was more lower than that in control group（P<0.05）,and the expression of β₂-AR in β₂-AR antagonist group was more higher than that in control group（P<0.05）. Conclusion β₂-AR can express in bone marrow cells of normal mice, and the expression level of β₂-AR is related to hematopoietic function of bone marrow,it may be involved in hematopoietic regulation by neuro-endocrine-immunity net work.

Key words: models, animal, receptors, adrenergic, beta-2