关键词: 聚乙二醇-聚乳酸-聚谷氨酸, 内皮细胞, 生物相容性材料

Abstract: Abstract:Objective To explore the cytocompatibility of biodegradable PEG-PLA-PGL/RGD ［pol（ethyleneglycol)-b-poly(l-lactide)-b-poly(l-glutamic acid）］ with human umbilical vein endothelial cells (HUVECs) and provide a basis for the development of biodegradable material for vessel scaffold. Methods The HUVESCs cultivated in vitro were inoculated onto PEG-PLA-PGL/RGD membrane and their growth and cell count were observed by phase contrast microscopy. Immunohistochemistry was used to examine the existence of factor Ⅷ expressed by HUVESs.The proliferation of cells was observed by MTT method.Results The observation result of phase contrast microscopy showed that endothelial cells grew well in PEG-PLA-PGL/RGD membrane and showed no significant difference with control. Cells attached to the PEG-PLA-PGL/ RGD and stretched after grafting 4—6 h.After 3 d,cells began to grow in colony rapidly.After 5 d,colony began to fuse and seemed like stone,the cells were shuttle and polygon in shape. Counting of HUVECs in 1 week: growth rate of cells in experimental group didn’〖KG-3〗t decrease significantly as compared with control（P>0.05）. There were no significant differences of the proliferation index of cells by MTT method 1, 3,5 and 7 d after inoculation between experimental and control groups (P>0.05). Conclusion Tissue engineering blood vessel with normal cytocompatibility to a certain degree can be constructed through seeding cultured HUVECs on PEG-PLA-PGL/RGD scaffold and PEG-PLA-PGL/RGD is a perfect biodegradable carrier material.

Key words: PEG-PLA-PGL, RGD, endothelial cells, biocompatible materials