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• 基础研究 • 上一篇    下一篇

四君子汤对运动后不同类型肌纤维作用的细胞化学变化

孙常义,王明辉,杨道宁,吴为宏   

  1. 吉林大学体育教学与研究中心,吉林 长春130025
  • 收稿日期:2005-07-14 修回日期:1900-01-01 出版日期:2006-07-28 发布日期:2006-07-28

Cytochemical changes of effect of Sijunzitang on different types of muscle fiber after exercise

SUN Chang-yi,WANG Ming-hui,YANG Dao-ning,WU Wei-hong   

  1. Center of Physical Education and Research, Jilin University, Changchun 130025,China
  • Received:2005-07-14 Revised:1900-01-01 Online:2006-07-28 Published:2006-07-28

摘要: 目的:探讨四君子汤对解除骨骼肌疲劳的作用。 方法:选择小鼠32只,分成安静组、疲劳组、自然恢复组和四君子汤组,后3组每天在水池中进行游泳运动50 min。四君子汤组腹腔注射四君子汤0.2 mL/只/日,其他各组给予同等剂量的生理盐水,在运动结束后各组动物立即处死同时取材。采用组织化学形态定量技术,运用MPV2型显微分光光度计对运动疲劳小鼠股四头肌不同类型(Ⅰ型、ⅡA型、ⅡB型)肌纤维的成分进行定量分析。 结果:耐力运动后疲劳组动物骨骼肌的糖原含量和琥珀酸脱氢酶(SDH)活性与安静组比较,显著降低(P<0.05或P<0.01);恢复后的四君子汤组的3种肌纤维类型的糖原含量、SDH活性明显高于疲劳组(P<0.05或P<0.01)。 结论:四君子汤对解除骨骼肌的疲劳、增强体质具有非常重要的作用。

关键词: 肌, 骨骼, 肌疲劳, 肌纤维, 组织细胞化学

Abstract: Objective To discuss the function of Sijunzitang on relieving the exhaustion fatigue of muscle. Methods Thirty-two mice were divided into Quiet group,Fatigue group,Nature recovery group and Sijunzitang group. The mice in last three groups swam in the pool for 50 min every day. During exercise 0.2 mL Sijunzitang was injected into each mouse in Sijunzitang group every day. And the same dosage of normal saline was injected into other groups. After exercise, all the mice were killed and taken as material immediately. The technique of cytochemistry and Type MPV2 microspectrophotometer were used to test quantitatively the chemical components of different types (Ⅰ type, ⅡA type and ⅡB type) of muscle fibers in the quadriceps of mice after exhaustion exercise. Results After exhaustion exercise the content of glycogen and the activity of SDH in the muscle fibers in Fatigue group reduced remarkably (P<0.05 or P<0.01) compared with Quiet group.The content of glycogen and the activity of SDH in the muscle fibers in Sijunzitang group was significantly higher than that in Fatigue group (P<0.05 or P<0.01). Conclusion Sijunzitang can relieve the exhaustion fatigue of muscle and build up health.

Key words: muscle, skeletal, muscle fatigue, muscle fibers, histocytochemistry

中图分类号: 

  • R282.71