关键词: 药理学, Hela细胞, 药物作用, 细胞周期, 药物作用

Abstract: Objective To discuss the effects of curcuma on different phases of the Hela cell proliferation in order to find the effective medicine in cervix cancer treatment. Methods MTT colorimetry and flow cytometry were used to measure the inhibitory rate of Hela cell proliferation and the changes of cell cycle, and transmission electron microscope (TEM) was used to observe the changes of the Hela subcells treated with the different concentrations of curcuma （0，10， 20 and 40 mg·L^-1）.　 Results Curcuma （0，10，20 and 40 mg·L^-1）had obvious inhibitory effects on the Hela cell proliferation in a dose-dependant manner，the inhibitory rates were 3.0%，21.4%，32.8% and 49.2%，respectively. Furthermore, flow cytometry showed that the number of cells in G₁ phase increased and the number of cells in S phase decreased, the number of cells in G₂／M phases relatively increased. The changes of subcell structure could be seen, such as cavernous cells, cytoplasm agglutination, increasing apoptosis. Conclusion Curcuma can inhabit the Hela cell proliferation, prevent the cells in G₁ phase from entering into S phase, and promote Hela cell apoptosis.

Key words: pharmacology, Hela cells, drug effects, cell cycle, drug effects