关键词: 心肌缺血再灌注损伤, 心肌酶, 自由基, 血管活性物质, 血小板聚集功能

Abstract: Objective To observe the protective effects of Ginsenoside-Rb(G-Rb) on experimental myocardial ischemia-reperfusion injury in rats.Methods The myocardial ischemia-reperfusion model was induced by 30 min left anterior descending coronary occulusion and 24 h reperfusion in openchest anesthetized rat.The changes of aspartate aminotransferase (AST),lactate dehydrogenase (LDH),MB isoenzyme of creatine kinase(CK-MB),superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and malondialdehyde(MDA) content in serum,and prostacycline(PGI₂) and thromboxane A₂ (TXA₂) levels in plasma were determined.At the same time,the activity of platelet aggregation was also determined.Results In rats treated with G-Rb (with dose of 50 and 100 mg•kg^-1 i.g for 7 d),the MIS was significantly reduced (P<0.05 or P<0.01),the AST,LDH and CK-MB activities in serum and the TXA₂ level in plasma were declined (P<0.05 or P<0.01),while PGI2 level in plasma and PGI₂/TXA₂ ratio were increased signficatly (P<0.05 or P<0.01).In addition,the LPO content in serum was declined,SOD and GSH-Px activities in serum were increased markedly (P<0.05 or P<0.01).The platelet aggregation rate(PAR) at 1,3 and 5 min and maximum platelet aggregation rate(MOAR) were decreased markedly (P<0.05 or P<0.01).Conclusion G-Rb has protective effects on myocardial ischemia-reperfusion injury through improving free radicals metabolism,decreasing TXA₂ level in plasma,increasing PGI₂ level in plasma and PGI₂/TXA₂ ratio,and inhibitting the activity of platelet aggregation etc.

Key words: myocardial ischemia-reperfusion injury, myocardialenzyme, free radicals, vasoactive factors, platelet aggregation function