关键词: 抗原, CD80, 辐射, 电离, 基因疗法, 肿瘤, 实验性

Abstract: Objective To study the inhibitory effects of gene combined radiotherapy on mice transplanted with B16 melanoma.Methods Alkaline lysis assay was used to extract and purify the plasmid. Plasmid DNA was injected into tumor by microinjection assay. Mice were inoculated with 5×10⁵ of B16 melanoma in right hind legs and the therapy was performed when the diameter of tumor reached at 0.3-0.5 cm. pNE-mIL-12 and pcDNA-B7-1 plasmids were injected locally three times following with irradiation three times. The tumor growth rate of mice was observed. Results The anti-tumor effect of pNE-mIL-12 combined with pcDNA-B7-1 plasmid following with 2 Gy X-ray irradiation was much better than other groups. It showed that the tumor growth rate was slowed, the survival days of mice were delayed significantly (P<0.01,P<0.001), the death rate of mice 31 d after therapy was only 22.2% and the tumor was deleted in one mouse. Conclusion Multi-genes combined with radiotherapy could inhibit the tumor growth of mice inoculated B16 melanoma and the inhibitory effect is better than single gene therapy and radiotherapy.

Key words: antigens, CD80, radiation, ionizing gene therapy, neoplasms, experimental