关键词: 电离, 脾细胞, 巨噬细胞, 细胞凋亡

Abstract: Objective To study the effects of whole-body irradiation（WBI）with different doses of X-rays on apoptosis in mouse splenocytes and peritoneal macrophages. Methods The apoptosis percentages of mouse splenocytes and peritoneal macrophaes were detected with flow cytometry（FCM）at different time after the whole-body X-irradiation using the staining of Annexin-V and PI. Results As com pared with the control，the percentage of apoptosis in mouse splenocytes began to increase gradually 24 h after WBI with 2 Gy X-rays（Ｐ<0.05），and the percentage of apoptosis in mouse peritoneal macrophages aros e 4 h after 2 Gy irradiation,and kept higher level till 48 h（Ｐ<0.05，Ｐ<0.01，Ｐ<0.001）. After WBI with 0.075 Gy X-rays，the percentage of apoptosis in mouse splenocytes reduced at 24 h （Ｐ<0.05），and the percentage of apoptosis in peritoneal macrophages significantly reduced from 2 h to 16 h（Ｐ<0.05）as compared with the control. Conclusion High dose irradiation can stimulate apoptos is in mouse splenocytes and peritoneal macrophages， while low dose irradiation can cause an opposite effect and even suppress immunocyte apoptosis.

Key words: ionizing, splenocytes, macrophages, apoptosis