关键词: 癌, 肝细胞, 细胞周期

Abstract: Objective To observe the inhibitory effect of LaCl₃ on the growth of human hepatocellular carcinoma cell lines SMMC-7721 and its mechanism. Methods The cells were treated with different doses of LaCl₃ and the growth curve, colony inhibitory rates,the levels of AFP of cultured cells were detected at 1,3 , 5 and 7 days after they were dealed withLaCl₃. The changes of cell cycle were detected by flow cytometry and the expressions of cell cycle protein CyclinD₁,PCNA,CDK₄ and P16 were observed by immunocytochemistry staining . Results MTT test showed tha t SMMC-7721 cell proliterating activity was obviously inhibited by LaCl₃ in a time and dose related manner at concentrations of 0.50 and 1.00 mmol•L^-1. The colony forming inhibitory rate of 0.10, 0.50 and 1.00 mmol•L^-1 LaCl₃ were 51%, 67% and 97% （P<0.05, P<0.01 ）.Cell cycle progress of SMMC-7721 changed obviously when treated with different does of LaCl₃ ,the percent of G₀/G₁ phase was higher and S phase lower, the difference was significant compared with control group（P<0.01). 0.10,0.50 and 1.00 mmol•L^-1 LaCl₃ could decreased the levels of AFP, the effect of 1.00 mmol•L^-1 LaCl₃ was notable（P<0.01 ）.Immunocytochemist ry showed that LaCl₃ could enhance the expression of P16 protein and decrease the expressions of CyclinD₁,PCNA and CDK₄. Conclusion LaCl₃ could inhabit the growth of SMMC-7721 cell and its mechanism might be connected with the inhibiti on of cell cycle progress and decrease the degree of malignancy.

Key words: carcinoma, hepatocellular, cell cycle