虾青素对凹透镜所致的豚鼠近视模型屈光状态及病理组织形态的影响

doi:10.13481/j.1671-587x.20170514

吉林大学学报(医学版) ›› 2017, Vol. 43 ›› Issue (05): 932-936.doi: 10.13481/j.1671-587x.20170514

虾青素对凹透镜所致的豚鼠近视模型屈光状态及病理组织形态的影响

石晶, 谭小波, 杨洁, 郝佳颖, 杨伟丽, 李娜

承德医学院附属医院眼科, 河北承德 067000

收稿日期:2016-12-17 出版日期:2017-09-28 发布日期:2017-09-29
通讯作者: 谭小波,副主任医师,硕士研究生导师(Tel:0314-2279415,E-mail:tanxiaobifirst@163.com) E-mail:tanxiaobifirst@163.com
作者简介:石晶(1979-),女,河北省承德市人,副主任医师,医学硕士,主要从事儿童眼病、斜弱视和眼屈光方面的研究。
基金资助:
河北省科技厅自然科学基金资助课题（H2015406054）；河北省承德市科技局自筹经费项目资助课题（201121058）

Influence of astaxanthin in refractive status and pathohistology of myopia models caused by concave lens of guinea pigs

SHI Jing, TAN Xiaobo, YANG Jie, HAO Jiaying, YANG Weili, LI Na

Department of Ophthalmology, Affiliated Hospital, Chengde Medical School, Chengde 067000, China

Received:2016-12-17 Online:2017-09-28 Published:2017-09-29

摘要/Abstract

摘要： 目的：探讨虾青素对凹透镜所致的豚鼠近视模型屈光状态及病理组织形态的影响，阐明虾青素对近视屈光状态的改善作用。方法：48只健康豚鼠随机分为空白对照组（n=12）和模型组（n=36），模型组以远视性光学离焦法制备豚鼠右眼近视模型（左眼作为对照）后随机分为模型对照组、低（25 ig·kg^-1）和高剂量虾青素组（50 ig·kg^-1），给药干预4周，于2和4周分别检测各组豚鼠双眼屈光度和眼轴长度，并于4周检测完成后，对右眼进行病理组织形态学检查。结果：模型组豚鼠在实验2周后实验眼球屈光度较作为对照的左眼低（P < 0.05），与空白对照组右眼比较也明显降低（P < 0.05），4周后更加明显（P < 0.01）；模型组豚鼠在实验2周后实验眼球的眼轴长度较对照的左眼及空白对照组增加（P < 0.05），4周后增加更为明显（P < 0.01）。与模型组比较，虾青素组豚鼠眼屈光度及眼轴长度明显增加（P < 0.05），用药时间长且效果较好；其病理组织形态学上也呈现出明显的改善，胶原断裂现象明显减少且排列较整齐。结论：虾青素在改善近视眼屈光度及眼轴长度方面有良好的效果，还有助于恢复近视眼球巩膜的组织形态。

关键词: 近视, 虾青素, 屈光度, 眼轴长度, 豚鼠, 疾病模型, 动物

Abstract: Objective: To investigate the effect of astaxanthin on the refractive status and pathological morphology of the guinea pig myopia models induced by concave lens, and to elucidate the improvement effect of astaxanthin on the refractive status of myopia. Methods: A total of 48 healthy guinea pigs were randomly divided into blank control group(n=12) and model group(n=36);the guinea pig myopia models(right eyes) were established with far vision optical method(left eyes were used as control).The guinea pig models were divided into model control group, high of astaxanthin (50 ig·kg^-1) group and low dose of astaxanthin(25 ig·kg^-1)group. After 2 and 4 weeks treatment, the diopters and the lengths of optic axis of both eyes of each guinea pig in various groups were tested, and at 4 weeks the right eyes of the pigs were removed and the pathomorphology was observed by HE staining. Results: The diopters of the model eyes in model group were lower than those of left eyes used as control(P<0.05) and those of right eyes in blank control group after 2 and 4 weeks (P<0.05 or P<0.01).The length of optic axis of the model eye in model group was increased compared with left eye used as controls and blank control group after 2 weeks (P<0.05) and especially at 4 weeks(P<0.01). Compared with model group,the axial oculi and diopters of the guinea pigs in astaxanthin groups were increased (P<0.05);the pathohistological changes were improved,the collagen fracture was obviously decreased,and the collagen fibers were orderly arranged. Conclusion: Astaxanthin can recover the length of optic axis, diopter and histomorphology of sclera of guinea pig myopia models in some extents.

Key words: astaxanthin, myopia, diopter, length of optic axis, guinea pig, disease model, animanl

中图分类号:

R778

石晶, 谭小波, 杨洁, 郝佳颖, 杨伟丽, 李娜. 虾青素对凹透镜所致的豚鼠近视模型屈光状态及病理组织形态的影响[J]. 吉林大学学报(医学版), 2017, 43(05): 932-936.

SHI Jing, TAN Xiaobo, YANG Jie, HAO Jiaying, YANG Weili, LI Na. Influence of astaxanthin in refractive status and pathohistology of myopia models caused by concave lens of guinea pigs[J]. Journal of Jilin University Medicine Edition, 2017, 43(05): 932-936.

参考文献

[1] Recko M, Stahl ED.Childhood myopia:epidemiology, risk factors, and prevention[J]. Mo Med,2015,112(2):116-121.
[2] Aller TA. Clinical management of progressive myopia[J]. Eye (Lond),2014,28(2):147-153.
[3] Walline JJ. Myopia Control:A Review[J]. Eye Contact Lens,2016,42(1):3-8.
[4] 陶姝颖,明建.虾青素的功能特性及其在功能食品中的应用研究进展[J].食品工业,2012,33(8):110-116.
[5] Ono T, Kamimura N, Matsuhashi T, et al. The histone 3 lysine 9 methyltransferase inhibitor chaetocin improves prognosis in a rat model of high salt diet-induced heart failure[J]. Sci Rep, 2017,7:39752.
[6] Wang F,Zhou J,Lu Y, et al. Effects of 530 nm green light on refractive status,melatonin, MT1 receptor, and melanopsin in the guinea pig[J]. Curr Eye Res, 2011,36(2):103-111.
[7] Jones-Jordan LA, Sinnott LT, Graham ND, et al. The contributions of near work and outdoor activity to the correlation between siblings in the collaborative longitudinal evaluation of ethnicity and refractive error (CLEERE) study[J]. Invest Ophthalmol Vis Sci,2014, 55(10):6333-6339.
[8] 查屹,陈瑶瑶,蔡剑秋. 高度近视遗传因素及基因研究进展[J]. 实用医学杂志,2011,27(8):1495-1496.
[9] Tideman JW, Fan Q, Polling JR, et al. When do myopia genes have their effect? Comparison of genetic risks between children and adults[J]. Genet Epidemiol, 2016,40(8):756-766.
[10] Wilkinson JT, Songy CE, Bumpass DB,et al. Curve modulation and apex migration using shilla growth guidance rods for early-onset scoliosis at 5-year follow-up[J]. J Pediatr Orthop,2017.doi:10.1097/BPO.0000000000000983[Epub ahead of print].
[11] Al-Amin MM, Rahman MM, Khan FR, et al. Astaxanthin improves behavioral disorder and oxidative stress in prenatal valproic acid-induced mice model of autism[J]. Behav Brain Res,2015,286:112-121.
[12] 钟雨彤.虾青素的生理功能与应用领域[J].科技展望,2016,26(23):290.
[13] Sabour-Pickett S, Nolan JM, Loughman J,et al. A review of the evidence germane to the putative protective role of the macular carotenoids for age-related macular degeneration[J]. Mol Nutr Food Res,2012,56(2):270-286.
[14] Ghlissi Z, Hakim A, Sila A, et al. Evaluation of efficacy of natural astaxanthin and vitamin E in prevention of colistin-induced nephrotoxicity in the rat model[J]. Environ Toxicol Pharmacol,2014,37(3):960-966.
[15] 杨艳,周宇红,徐海滨,等. 虾青素抗氧化作用动物实验研究[J]. 现代预防医学, 2009, 36(13):2432-2433.

虾青素对凹透镜所致的豚鼠近视模型屈光状态及病理组织形态的影响

Influence of astaxanthin in refractive status and pathohistology of myopia models caused by concave lens of guinea pigs

RichHTML

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	白洁, 纪文静, 丁永年, 彭媛媛, 陈源文. 胆总管结扎致肝纤维化模型大鼠肝脏内源性AcSDKP及其调控因子水平的变化[J]. 吉林大学学报(医学版), 2018, 44(05): 999-1004.
[2]	刘洋, 怀施涛, 付文博, 宋亚芹, 卢沐, 庞丽娟, 魏育涛. 冠心病伴心外膜脂肪增厚大鼠模型的制备[J]. 吉林大学学报(医学版), 2017, 43(01): 176-180.
[3]	石立强, 李洪宇, 苑广信, 糜心雅, 谢立亚, 杜培革, 安丽萍. 高活性小牛血去蛋白提取物对四氯化碳致急性肝损伤模型小鼠的保护作用[J]. 吉林大学学报(医学版), 2017, 43(01): 32-35.
[4]	陈阳, 鄂长勇, 关连越, 刘文韬, 王丽娜, 张学文. 四氯化碳诱导的肝硬化小鼠肝部分切除后肝再生模型的建立及评价[J]. 吉林大学学报(医学版), 2016, 42(06): 1243-1248.
[5]	丁显飞, 周学良, 豆萌萌, 李砺锋, 郭丽娜, 王峰, 樊青霞. 哺乳动物雷帕霉素靶蛋白的表达及其与乳腺癌预后关系的Meta分析[J]. 吉林大学学报(医学版), 2016, 42(04): 783-788.
[6]	宋志强, 沈海山, 沈俊, 吴建臣, 张明, 李然伟, 张继珍, 李胜文. MNU法诱导大鼠原位膀胱癌模型的建立及MRI的诊断价值[J]. 吉林大学学报(医学版), 2016, 42(04): 685-689.
[7]	张宸豪, 李瑶, 陈为, 赵良中, 李妍. FTY720对实验性肝纤维化小鼠肝损伤的保护作用[J]. 吉林大学学报(医学版), 2015, 41(06): 1154-1157.
[8]	何婧, 戚迪, 王导新. 胰岛素通过mTORC2/SGK1途径上调肺泡上皮钠通道α亚基的作用机制[J]. 吉林大学学报(医学版), 2015, 41(04): 716-720.
[9]	任国君，孙杰，申风娟，史春红，于涛，姜如娇，吴英杰，孙捷. 胰腺β细胞生长激素受体基因敲除对STZ诱发1型糖尿病小鼠的影响[J]. 吉林大学学报(医学版), 2014, 40(04): 753-756.
[10]	周颖，袁博，马越，张琨，高峰，王皓，芦晋，吴晓刚，佟丹，袁长吉 . C6脑胶质瘤模型大鼠MRI影像及形态学观察及其意义[J]. 吉林大学学报(医学版), 2013, 39(2): 379-382.
[11]	曾晨光,周彦恒,林久祥,丁鹏 . 不同加载条件下国产微螺钉种植体的支抗稳定性及组织学表现[J]. 吉林大学学报(医学版), 2013, 39(2): 300-303.
[12]	刘国民\|孔宁\|韩宏志\|于海涛\|张琪\|孙红辉. 鹿、羊和人腰椎生物学参数的比较及其意义[J]. J4, 2012, 38(6): 1096-1100.
[13]	宋建明\|黄秀清\|林燕惠\|张敏. 变应性鼻炎大鼠模型的建立[J]. J4, 2012, 38(6): 1152-1155.
[14]	周金影\|曲绍春\|于晓风\|王佳\|睢大筼. “肾阳虚证”下心肌梗死模型与单纯心肌梗死模型大鼠心肌形态学、心肌酶学及血液流变学的比较[J]. J4, 2012, 38(3): 471-476.
[15]	吴冰\|康劲松\|王贺元\|晞欣\|邱海洋\|王伟伟\|罗祺. 枕大池二次注血法建立大鼠蛛网膜下腔出血模型及尼莫地平的脑保护作用[J]. J4, 2011, 37(6): 1153-1156.