吉林大学学报(医学版) ›› 2017, Vol. 43 ›› Issue (05): 928-931.doi: 10.13481/j.1671-587x.20170513

• 基础研究 • 上一篇    下一篇

北五味子多糖对脂多糖诱导中性粒细胞炎症的抑制作用

孙晓红1, 李嘉仪1, 李洪涛1, 谢宇2, 孙新2, 孔繁利2   

  1. 1. 北华大学基础医学院病原生物学教研室, 吉林 吉林 132013;
    2. 吉林省分子老年医学重点实验室, 吉林 吉林 132013
  • 收稿日期:2017-03-09 出版日期:2017-09-28 发布日期:2017-09-29
  • 通讯作者: 孔繁利,副教授,医学博士,硕士研究生导师(Tel:0432-64608016,E-mail:kongfanli5@163.com) E-mail:kongfanli5@163.com
  • 作者简介:孙晓红(1968-),女,吉林省吉林市人,副教授,医学博士,主要从事病原生物学及中药开发方面的研究。
  • 基金资助:
    吉林省科技厅科研基金资助课题(20130624003JC,20150312013ZG);吉林省教育厅科学技术研究项目资助课题(2014201,201664);北华大学省级大学生创新项目资助课题(2016-199100282);北华大学博士启动基金资助课题(199500031)

Inhibitory effect of north-Schisandra chinensis polysaccharide on neutrophil inflammation induced by lipopolysaccharide

SUN Xiaohong1, LI Jiayi1, LI Hongtao1, XIE Yu2, SUN Xin2, KONG Fanli2   

  1. 1. Department of Pathogenic Biology, College of Basic Medical Sciences, Beihua University, Jilin 132013, China;
    2. Key Laboratory of Molecular Geriatric Medicine of Jilin Province, Jilin 132013, China
  • Received:2017-03-09 Online:2017-09-28 Published:2017-09-29

摘要: 目的:探讨满药北五味子多糖(NSCP)对脂多糖(LPS)激活的离体培养人中性粒细胞的影响,阐明其抗炎作用机制。方法:利用LPS处理人中性粒细胞建立炎症细胞模型,实验分为对照组、LPS组(1.0 mg·L-1)及NSCP组(1.25、2.50和5.00 g·L-1),NSCP组先加入LPS刺激60 min,再加入不同浓度NSCP。应用酶联免疫吸附法(ELISA)检测中性粒细胞中肿瘤坏死因子α(TNF-α)水平,采用流式细胞术检测中性粒细胞凋亡率。结果:与对照组比较,LPS组TNF-α水平升高(P < 0.05);与LPS组比较,NSCP组TNF-α水平降低(P < 0.05)。LPS组中性粒细胞凋亡百分率较对照组降低(P < 0.05);NSCP组中性粒细胞凋亡百分率随时间及剂量增加而升高,其中5.00 g·L-1NSCP作用16 h促进其凋亡作用最佳,与LPS组比较凋亡百分率明显升高(P < 0.05)。结论:NSCP可通过抑制LPS诱导TNF-α分泌及中性粒细胞凋亡延迟而发挥抗炎作用。

关键词: 北五味子多糖, 中性粒细胞, 肿瘤坏死因子α, 细胞凋亡

Abstract: Objective: To investigate the effect of Manchu medicine north-Schisandra chinensis polysaccharide (NSCP) on the human neutrophils treated by lipopolysaccharide (LPS) cultivated in vitro, and to elucidate its anti-inflammatory mechanism. Methods: The neutrophilic inflammatory cell model was established with LPS. The experiment included control group, LPS group (1.0 mg·L-1) and NSCP group (1.25, 2.50 and 5.00 g·L-1), the cells in NSCP group were first treated with LPS for 60 min, and then treated with different concentrations of NSCP. The levels of TNF-α in neutrophils were measured with ELISA and the apoptotic rates were detected by flow cytometry. Results: The level of TNF-α in LPS group was increased compared with control group (P<0.05). The level of TNF-α in NSCP group was decreased compared with LPS group (P<0.05).The apoptotic rate in LPS group was decreased compared with control group (P<0.05);the apoptotic rates in NSCP group were increased with the increasing of time and dose, and the best effect was found 16 h after treatment with 5 g·L-1NSCP;the apoptotic rate in NSCP group was significantly increased compared with LPS group (P<0.05). Conclusion: NSCP can perform the anti-inflammation effect through the suppression of LPS-induced TNF-α secretion in neutrophils and the promotion of neutrophils apoptosis.

Key words: tumor necrosis factor-α, north-Schisandra chinensis polysaccharide, neutrophil, cell apoptosis

中图分类号: 

  • R285.5