关键词: sub>基因, 白血病, 聚合酶链反应, 突变

Abstract: Objective To investigate Flt₃ tandem duplication mutation (ITD) and its distribution in different malignant hematopoietic diseases. Methods PCR was used to detect the Flt₃-ITD mutation in blast cell of bone marrow from 103 patients with malignant hematopoietic disease. Results Flt₃-ITD mutations were found in 18.2% （8/44）of the patients with primary acute myeloid leukemia (AML), more Flt₃ aberrations were found in patients with FAB M1. Flt₃-ITD mutations were not found in patients with acute lymphoid leukemia (ALL), myelodysplastic syndrome(MDS), multiple myeloma(MM), non-Hodgkin′s lymphoma(NHL), and chronic myeloid leukemia (CML). Conclusion Flt₃-ITD can be a important molecular marker for AML.

Key words: sub> gene, leukemia, polymerase chain reaction, mutation