自身免疫调节因子对CD4+CD25+Treg细胞的影响

J4 ›› 2010, Vol. 36 ›› Issue (5): 866-870.

自身免疫调节因子对CD4+CD25+Treg细胞的影响

杨巍|朱武飞|和知非|魏晓曦|苏静波|李一

吉林大学基础医学院免疫学教研室|吉林长春 130021

收稿日期:2010-04-15 出版日期:2010-09-28 发布日期:2010-09-28
通讯作者: 李一（Tel：0431-85619476，E-mail：yilili19@yahoo.com.cn） E-mail:yilili19@yahoo.com.cn
作者简介:杨巍（1980-）|女|吉林省长春市人|讲师|医学博士|主要从事免疫耐受机制研究。
基金资助:
国家自然科学基金资助课题（30872307）

Effects of autoimmune regulator on CD4+CD25+Treg cells

YANG Wei, ZHU Wu-Fei, HE Zhi-Fei, WEI Xiao-Xi, SU Jing-Bo, LI Yi

Department of Immunology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China

Received:2010-04-15 Online:2010-09-28 Published:2010-09-28

摘要/Abstract

摘要：

目的：观察自身免疫调节因子（AIRE）对CD4+CD25+Treg细胞的影响，探讨其在外周免疫耐受中的作用及机制。方法：pEGFPC3-AIRE质粒通过脂质体介导转染RAW264.7细胞；通过流式细胞术检测CD4+CD25+Treg细胞的数量；采用半定量RT-PCR法检测Foxp3和转化生长因子β（TGF-β）mRNA的表达；采用ELISA法检测TGF-β1水平。结果：AIRE转染的RAW264.7细胞可直接或间接诱导CD4+CD25+Treg细胞的产生及Foxp3 mRNA表达增强(P<0.05)。结论：表达在外周抗原提呈细胞（APCs）上的AIRE可直接和（或）间接影响CD4+CD25+Treg细胞的诱导产生及功能，从而可能在外周免疫耐受中发挥重要作用。

关键词: 自身免疫调节因子；CD4+CD25+Treg细胞；免疫耐受

Abstract:

Abstract:Objective To observe the effects of autoimmune regulator(AIRE) on CD4+CD25+Treg cells,and explore the functions and mechanisms of AIRE in peripheral tolerance.Methods RAW264.7 cells were transfected with pEGFPC3AIRE plasmid via lipofectin.Flow cytometry was used to determine the amount of CD4+CD25+Treg cells. Then semi-quantitative RT-PCR was employed to detect the expressions of Foxp3 and TGF-β1 mRNA.The level of TGF-β1 was detected with ELISA kit. Results AIRE transfected RAW264.7 cells could directly or indirectly induce the generation of CD4+CD25+Treg cells and the increased expression of Foxp3 mRNA.Conclusion The AIRE expressed in the peripheral antigen presenting cells(APCs) could directly and/or indirectly induce the generation and functions of CD4+CD25+Treg cells,and probably plays a crucial role in the peripheral immune tolerance.

Key words: autoimmune regulator, CD4+CD25+Treg cell, immune tolerance

中图分类号:

R392.1

杨巍, 朱武飞, 和知非, 魏晓曦, 苏静波, 李一. 自身免疫调节因子对CD4+CD25+Treg细胞的影响[J]. J4, 2010, 36(5): 866-870.

YANG Wei, ZHU Wu-Fei, HE Zhi-Fei, WEI Xiao-Xi, SU Jing-Bo, LI Yi. Effects of autoimmune regulator on CD4+CD25+Treg cells[J]. J4, 2010, 36(5): 866-870.