外源硫酸锌对糖尿病大鼠肝脏损伤的保护作用

J4 ›› 2010, Vol. 36 ›› Issue (5): 871-874.

外源硫酸锌对糖尿病大鼠肝脏损伤的保护作用

卢应¹|吴文杰²|刘娅³|李鸿雁³|张桂英³|王雪³

1.吉林大学第一医院ICU科|吉林长春 130021；2.浙江省舟山市疾病预防控制中心免疫规划科|浙江舟山 316021；3.吉林大学公共卫生学院营养与食品卫生学教研室|吉林长春 130021

收稿日期:2010-06-12 出版日期:2010-09-28 发布日期:2010-09-28
通讯作者: 刘娅 (Tel: 0431-85166352, E-mail:liuya@jlu.edu.cn) E-mail:liuya@jlu.edu.cn
作者简介:卢应 (1976-),女,吉林省长春市人,主治医师|在读医学博士,主要从事糖尿病并发症的研究。
基金资助:
国家自然科学基金资助课题（30370669）

Protective effect of exogenous zinc sulfate on liver injury of diabetic rats

LU Ying¹, WU Wen-Jie², LIU Ya³, LI Hong-Yan³, ZHANG Gui-Ying³, WANG Xue³

. Department of ICU, First Hospital, Jilin University, Changchun 130021, China 2. Center for Disease Control and Prevention of Zhoushan City|Zhoushan 316021, China|3. Department of Nutrition and Food Hygiene, School of Public Health, Jinlin University, Changchun 130021, China

Received:2010-06-12 Online:2010-09-28 Published:2010-09-28

摘要/Abstract

摘要：

目的：观察外源性硫酸锌对链脲佐菌素（STZ）诱导糖尿病大鼠模型肝脏组织损伤的保护作用并探讨其机制。方法：健康Wistar大鼠50只，随机分为正常对照组(n=9)、高脂对照组(n=10)、高脂糖尿病模型组(n=7)、高脂糖尿病锌干预组(n=8)和高脂糖尿病后锌干预组(n=8)。锌干预组以锌离子浓度15 mg?kg-1?d-1进行灌胃，其他组以相应体积蒸馏水灌胃。糖尿病模型建立8周末，测定肝功，计算肝脏指数，测定肝脏组织超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)。结果：与糖尿病模型组比较，外源性硫酸锌干预能明显降低糖尿病大鼠的肝脏指数（P<0.05）；血清AST、ALT 水平有下降趋势（P>0.05）；减少肝脏组织MDA水平，并显著增加SOD及GSH-Px水平（P<0.05）；锌干预组组间肝脏组织MDA及SOD水平比较差异有显著性（P<0.05）。结论：外源性硫酸锌能够降低糖尿病模型大鼠肝脏指数，并改善其肝功能损伤，其机制可能与硫酸锌改善肝脏过氧化状态有关。

关键词: 糖尿病；高脂血症；锌；肝脏；抗氧化

Abstract:

Abstract:Objective
To study the protective effect of zinc sulfate on liver injury of diabetic rats induced by STZ and explore its mechanism. Methods Fifty male Wistar rats were randomly divided into five groups: normal diet (NC) group, high fat diet (FC) group, diabetes (DM) group, Zn before the intervention of diabetes (ZnDM) group, Zn after the intervention of diabetes (DMZn) group. The rats in Zn groups were fed with Zn ions (Zn2+) 15　mg?kg-1?d-1 by giving ZnSO4 solution daily by gavage, the others were fed with distilled water by gavage. Eight weeks after STZ injection, the serum was collected to detect the function of liver, AST and ALT, and liver was taken for the measurement of liver index, SOD, MDA, and GSH-Px. Results Compared with DM group, Zn intervention could significantly reduce the liver index (P<0.05); the levels of AST and ALT in serum were decreased mildl (P>0.05); the level of MDA in liver tissues was markedly decreased and the levels of SOD and GSH-Px in liver tissue were significantly improved (P<0.05)； the levels of MDA and SOD in liver tissues had significant differences between the two groups of Zn intervention (P<0.05). Conclusion Exogenous zinc sulfate can decrease the liver index of diabetic rats, and make the liver function better. The mechanism of the protection of Zn may be related to the strengthening of anti-oxidation.

Key words: diabetes mellitus, hyperlipidemia, zinc, liver, anti-oxidation

中图分类号:

R587.1

卢应, 吴文杰, 刘娅, 李鸿雁, 张桂英, 王雪. 外源硫酸锌对糖尿病大鼠肝脏损伤的保护作用[J]. J4, 2010, 36(5): 871-874.

LU Ying, WU Wen-Jie, LIU Ya, LI Hong-Yan, ZHANG Gui-Ying, WANG Xue. Protective effect of exogenous zinc sulfate on liver injury of diabetic rats[J]. J4, 2010, 36(5): 871-874.

[1]	刘斌,徐景伟,刘晶晶,田晓丰,乔士兴. 糖尿病大鼠胃旁路手术前后抑胃肽水平的检测及其意义[J]. 吉林大学学报(医学版), 2013, 39(2): 294-299.
[2]	李建会，李云，任治兴，高影. 黄芪联合胰岛素对糖尿病大鼠胰岛素抵抗的作用及其机制[J]. 吉林大学学报(医学版), 2013, 39(2): 246-250.
[3]	楚洪波，李冬梅，石搏，黄可欣，辛暨丽. 大豆皂苷对糖尿病大鼠心肌组织中NOX4和p22phox表达的影响及其对心肌的保护作用[J]. 吉林大学学报(医学版), 2013, 39(2): 251-254.
[4]	孙文伟\|魏丽琴\|孙丽娟\|侯杰\|任磊\|周雪艳. 胰岛素释放试验和胰岛素样生长因子-Ⅰ检测在2型糖尿病诊断和治疗中的应用[J]. J4, 2011, 37(3): 538-541.
[5]	周煦\|孙亚东\|马彦. 氨基末端脑钠素前体与2型糖尿病自主神经病变的相关性分析[J]. J4, 2010, 36(6): 1139-1142.
[6]	曲萌, 赵丽艳, 李才, 李相军, 孙波, 魏海峰. 多（二磷酸腺苷-核糖）水解酶抑制剂单宁酸对糖尿病大鼠肾脏病变的影响及其机制[J]. J4, 2010, 36(6): 1026-1029.
[7]	吕晓艳, 王春艳, 孟昭杰, 王云晶, 李鸥, 陈立, 邵明柏, 杜红伟. 1型11β-羟基类固醇脱氢酶在实验性2型糖尿病大鼠模型骨骼肌中的表达及意义[J]. J4, 2010, 36(5): 875-878.
[8]	何明蕊, 董宇, 付彤, 孙珉丹. 锌制剂对糖尿病大鼠肾脏和肾脏组织中VEGF、MT及MMP-9表达的影响[J]. J4, 2010, 36(4): 656-659.
[9]	刘锐, 何成彦, 徐冬岩, 刘波. 男性2型糖尿病患者骨质疏松症相关因素分析[J]. J4, 2010, 36(2): 408-411.
[10]	贾玉玺, 姜日花, 张旗. 糖尿病患者阴道念珠菌定植状况分析[J]. J4, 2009, 35(4): 706-709.
[11]	罗萍, 顾华, 吴曼, 罗曼宇. 氟伐他汀对高糖诱导的大鼠系膜细胞ERK1/2和CTGF表达的调控 [J]. J4, 2009, 35(4): 624-630.