吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (01): 83-87.doi: 10.13481/j.1671-587x.20150116

• 基础研究 • 上一篇    下一篇

复方苦荞麦制剂对大鼠糖尿病心肌损伤的保护作用

勾向博1, 白静1, 郭静2, 韩淑英1   

  1. 1. 河北联合大学基础医学院药理教研室, 河北 唐山 063000;
    2. 河北联合大学实验研究中心, 河北 唐山 063000
  • 收稿日期:2014-05-06 发布日期:2015-01-30
  • 通讯作者: 韩淑英,教授,硕士研究生导师(Tel:0315-3725870,E-mail:shuyinghan59@126.com) E-mail:shuyinghan59@126.com
  • 作者简介:勾向博(1984-),女,河北省沧州市人,助教,医学硕士,主要从事心血管药理学方面的研究。
  • 基金资助:

    河北省科技厅科技计划项目资助课题(122077175D)

Protective effect of Fufang KuQiaomai on myocardial injury of diabetic rats

GOU Xiangbo1, BAI Jing1, GUO Jing2, HAN Shuying1   

  1. 1. Department of Pharmacology, School of Basic Medical Sciences, Hebei United University, Tangshan 063000, China;
    2. Medical Experimental and Research Center, Hebei United University, Tangshan 063000, China
  • Received:2014-05-06 Published:2015-01-30

摘要:

目的: 观察复方苦荞麦制剂对大鼠糖尿病心肌损伤的保护作用,阐明其作用机制。方法: 腹腔一次性注射链脲佐菌素(STZ)建立大鼠糖尿病模型。糖尿病大鼠分为模型组﹑复方苦荞麦制剂组和贝那普利组,同时设正常对照组,观察各组大鼠体征变化,测定体质量(BW)、心脏质量指数(HWI)和心功能指标[心率(HR)、左心室收缩压(LVSP)、左心室舒张期末压(LVEDP)、左心室内压最大上升和下降速率(±dp/dtmax)],检测空腹血糖(FBG)和血清甘油三脂(TG)、总胆固醇(TC)及心肌组织中丙二醛(MDA )含量、超氧化物歧化酶( SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性;光镜下观察各组大鼠心肌组织形态学改变。结果: 与正常对照组比较,模型组大鼠FBG、HWI、LVEDP、TG和TC升高(P<0.01),BW、LVSP、HR和±dp/dtmax下降(P<0.05或P<0.01);心肌组织MDA水平显著升高(P<0.01),而SOD和GSH-Px活性则显著降低(P<0.05或P<0.01)。与模型组比较,复方苦荞麦制剂组和贝那普利组大鼠FBG、HWI和LVEDP下降(P<0.05或P<0.01),BW、LVSP、HR和±dp/dtmax升高(P<0.05或P<0.01);心肌组织MDA水平显著降低(P<0.01),而SOD和GSH-Px活性则显著升高(P<0.05或P<0.01),但复方苦荞麦制剂组与贝那普利组大鼠上述各指标比较差异均无统计学意义(P>0.05)。大鼠心肌组织形态学,模型组大鼠心肌纤维紊乱,心肌细胞肿胀,而复方苦荞麦制剂组和贝那普利组大鼠心肌纤维紊乱和心肌细胞肿胀减轻,心肌细胞结构基本正常。结论: 复方苦荞麦制剂对大鼠糖尿病心肌损伤具有保护作用,可能与抑制心肌脂质过氧化反应有关。

关键词: 复方苦荞麦制剂, 糖尿病, 实验性, 心肌损伤, 丙二醛, 超氧化物歧化酶, 谷胱甘肽过氧化物酶

Abstract:

Objective To study the protective effect of Fufang KuQiaomai (FFKQ) on the myocardial injury of the diabetic rats, and to charity its mechanism. Methods The diabetic rat models were established by intraperitoneal injecting streptozotocin (STZ).The diabetic rats were divided into model group, FFKQ group and benazepril group;at the same time, normal control group was set up.The body weight (BW), cardiac index (HWI), heart rate (HR), left ventricular systolic blood pressure (LVSP), left ventricular diastolic pressure (LVEDP) and ±dp/dtmax of the rats in various groups were measured 8 weeks after treatment.The levels of fasting blood glucose (FBG), triglyceride (TG) and total cholesterol (TC) in serum were detected;the malondialdehyde(MDA), superoxide dismutase(SOD) and glutathion peroxidase(GSH-Px) activities in myocardium tissue were measured to evaluate lipid metabolism. Results Compared with normal control group, the FBG, HWI, LVEDP, TG, TC and MDA of the rats in model group were increased(P<0.01);the BW, LVSP, HR, ±dp/dtmax, SOD and PSH-Px activities of the rats in model group were decreased(P<0.05 or P<0.01).Compared with model group, the FBG, HWI, LVEDP and MDA of the rats in FFKQ and benazepril groups were decreased(P<0.05 or P<0.01);the BW, LVSP, HR, ±dp/dtmax, SOD and PSH-Px activities were increased(P<0.05 or P<0.01), but all the indexes above of the rats had no significant differences between FFKQ and benazepril groups(P>0.05).The morphology of myocardium tissue of the rats in model group exhibited that the cardiac muscle fibers was confused and the cardiac muscle cells were spargosis, but FFKQ and benazepril improved the morphology of myocardium tissue of the diabetic rats. Conclusion FFKQ exerts a certain protective effect on cardiac injury of the diabetic rats through inhibiting lipid metabolism.

Key words: Fufang KuQiaomai, diabetes mellitus,experiment, myocardial injury, malondialdehyde, superoxide dismutase, glutathion peroxidase

中图分类号: 

  • R285.5