关键词: 副作用, NIH3T3, 成纤维细胞, 辐射效应, 细胞凋亡, 细胞周期, 流式细胞术

Abstract: Objective To study the damage effect of moderate-wave ultraviolet (UVB) on fibroblast and to establish the models of cellular damage. Methods Murine fibroblast line, NIH3T3, were treated with different doses of moderate-wave UVB. MTT assay was performed to evaluate the cellular survival rate，flow cytometry was performed to detect the cell cycle，apoptosis and the production of H₂O₂. Results The cellular survival rates decreased significantly 24 h after treatment with different doses of UVB (from 0.2 to 3.0 kJ•m^-2) (P<0.05 or P<0.01)；while 1.5 kJ•m^-2 UVB induced the increase of the percentages of G₁ phase and the increase of apoptotic bodies 4,8,18, and 24 h after irridiation，simultaneously 8 h after irridation with 1.5 kJ•m^-2 UVB, the increase of H₂O₂ production was found (P<0.05). Conclusion The UVB might induce the G₁ phase arrest，the increase of apoptosis and production of H₂O₂ in fibroblast.

Key words: adverse effects, NIH3T3, fibroblasts, radiation effects, apoptosis, cell cycle, flow cytometry