关键词: 白细胞介素12, 白细胞介素13, 酶联免疫吸附测定, 信号转导

Abstract: Abstract:Objective To investigate the effect of IL-12 on mediator release from mast cells and the potential signal transduction pathways correspondingly.Methods P815 cells were challenged with various concentrations of IL-12.The supernatants were collected and analyzed by enzyme-linked immunosorbent assay(ELISA) to detect the quantity of released IL-13,IL-6 and histamine.The cells were treated and analyzed by cellular activation of signal ELISA(CASE) to detect phosphorylation of ERK and P38.Results After incubated with different doses of IL-12(0,1.0，10.0 and 100.0 μg•L^-1),the IL-13 release of P815 cells increased with the concentration of IL-12 compared with medium alone control (P<0.05);but there were no significant differences of IL-6 release and histamine release compared with mediun alone control.After P815 cells were incubated with PD98059 and U0126 prior to IL-12,the percentage of intracellular phosphorylation of ERK and IL-13 release decreased significantly compared with groups without inhibitor (P<0.05) .After P815 cells were incubated with SB203580 prior to IL-12,the percentage of intracellular phosphorylation of P38 and IL-13 release didn’thange compared with groups without inhibitor.Conclusion IL-12 induced IL-13 release from P815 cells is likely through activation of ERK signalling pathway.

Key words: interleukin-12, interleukin-13, enzyme-linked immunosorbent assay, signal transducing