J4 ›› 2012, Vol. 38 ›› Issue (5): 981-984.

• 临床研究 • 上一篇    下一篇

实时荧光定量PCR法检测EG-1在甲状腺乳头状癌组织中的表达及其临床意义

孙亚东|张景斌|杨 乐|马 彦|姜春浩| 邸 军   

  1. 吉林省人民医院内分泌一疗区,吉林 长春 130021
  • 收稿日期:2012-04-10 出版日期:2012-09-28 发布日期:2012-09-28
  • 通讯作者: 孙亚东(Tel:0431-85595185,E-mail:ydsun-2005@163.com) E-mail:ydsun-2005@163.com
  • 作者简介:孙亚东 (1964-)|女|吉林省长春市人|主任医师|医学博士|主要从事内分泌代谢病基础及临床研究。
  • 基金资助:

     吉林省科技厅自然科学基金资助课题(201015127)

Expression |of EG-1 in human thyroid papillary carcinoma tissue detected by real-time quantitative 
PCR and its clinical significance

SUN Ya-dong, ZHANG Jing-bin, YANG Le,MA Yan, |JIANG Chun-hao,DI |Jun   

  1. Department of Endocrinology|People’s Hospital of Jilin Province, Changchun 130021,China
  • Received:2012-04-10 Online:2012-09-28 Published:2012-09-28

摘要:

目的:通过实时荧光定量PCR法检测内皮衍生基因-1(EG-1)的基因在甲状腺癌组织中的表达,探讨EG-1与甲状腺癌发生、发展的关系。 方法:选取38例甲状腺乳头状癌患者(病例组)和15例良性甲状腺疾病患者的甲状腺组织(对照组),采用实时荧光定量PCR法检测甲状腺组织中EG-1基因的表达,同时应用免疫组化学法检测EG-1的表达水平。结果:免疫组织化学结果显示, EG-1主要定位于癌细胞的细胞质,甲状腺乳头状癌组织中EG-1 mRNA和蛋白表达水平明显高于良性甲状腺疾病患者 (P<0.05)。EG-1 mRNA表达与组织学分级有关(P<0.05),与性别、年龄无明显关联性(
P>0.05)。结论:EG-1基因在甲状腺乳头状癌组织中表达明显增高,EG-1基因可能在甲状腺癌的发生、发展中起重要作用。

关键词: 实时荧光定量PCR, 内皮衍生基因-1;甲状腺肿瘤

Abstract:

Objective  To detect  the expression of endothelial derived gene-1 (EG-1) gene  in thyroid papillary carcinoma tissue by real-time quantitative PCR and to investigate  the relationship between EG-1 and the ocurrence and  development of thyroid papillary carcinoma. Methods Real-time quantitative PCR was used to detect
the expressions of EG-1 gene in 38 thyroid papillary carcinoma patients ( case group) and 15 thyroid adenoma patients (control group),and the expression level of EG-1 was detected by immunohistochemisty.
Results The expressions of EG-1 mRNA and protein in thyroid papillary carcinoma tissue  were significantly higher than those in adenoma  tissue(P<0.05).The expression of EG-1 mRNA had significant relationship with the pathological degree in thyroid cancer(P<0.05), but there was no correlation with the age and the gender of the patients(P>0.05).Conclusion The expression of EG-1 gene in thyroid papillary carcinoma tissue is obviously higher,EG-1 gene  may be important in the occurrence and  development of thyroid cancer.

Key words: real-time quantitative polymerase chain reaction, endothelial derived gene-1, thyroid neoplasms

中图分类号: 

  • R736.1