关键词: 单核细胞, 巨噬细胞, T淋巴细胞亚群

Abstract: Objective To study the effects of CD4⁺ T lymphocytes on processing mononuclear phagocyte in patients with asthma. Methods The mononuclear phagocytes and CD4⁺ T lymphocytes were isolated from peripheral blood in 20 patients with asthma and 22 healthy donors, respectively. Every sample was divided into four groups:MΦA⁺，(MΦCD4⁺)A⁺，MΦ⁺CD4⁺A⁺，and MΦA⁺CD4⁺. All groups were stimulated with CTLL₂P antigen for 18 hours and CTLL₂P antigen were washed away, then they were cultured with B lymphocytes together, the levels of secreted CTLL₂P-specific IgM, IgG, and IgE in the supernatant were measured after 10 days. Results When CD4⁺ T lymphocytes were cultured with mononuclear phagocytes together, the level of CTLL₂ P-specific IgM of patients with asthma (A₄₉₀ nm)（0.033±0.031） was lower than that of healthy donors（0.098±0.062）(P<0.05); the level of CTLL₂ P-specific IgG of patients with asthma (A₄₉₀ nm)（0.080±0.035）was significantly higher than that of healthy donors（0.055±0.024）(P<0.05)；the same result was obstained (P<0.05), when primate CD4⁺ T lymphocytes were cultured with antigen processing mononuclear phagocytes (patients:0.075±0.018,healthy donors:0.057±0.018). The CTLL₂ P-specific IgE level of patients with asthma(0.154±0.119) was as same as healthy donors (0.186±0.101)(P>0.05). Conclusion The CD4⁺ T lymphocytes of patients with asthma have different regulatory functions on immunolglobulins from antigen processing mononuclear phagocytes.

Key words: monocytes, macrophages, T-lymphocytes subsets