男性人群中载脂蛋白E基因多态性与血脂水平的关联性分析

doi:10.13481/j.1671-587x.20150427

吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (04): 808-813.doi: 10.13481/j.1671-587x.20150427

男性人群中载脂蛋白E基因多态性与血脂水平的关联性分析

傅倩晰¹, 周莉¹, 唐敏², 张名均³, 唐晓君¹

1. 重庆医科大学公共卫生与管理学院流行病学教研室, 重庆 400016;
2. 重庆医科大学检验医学院, 重庆 400016;
3. 重庆市九龙坡区第一人民医院检验科, 重庆 400050

收稿日期:2014-09-29 发布日期:2015-08-01
通讯作者: 唐晓君,副教授,硕士研究生导师(Tel:023-68485008,E-mail:tangxiaoj0726@qq.com) E-mail:tangxiaoj0726@qq.com
作者简介:傅倩晰(1988-),女,重庆市人,医学硕士,主要从事分子流行病学方面的研究。
基金资助:
国家自然科学基金资助课题(81202274)

Analysis on association between polymorphism of apolipoprotein E genotypes and blood lipid levels in male population

FU Qianxi¹, ZHOU Li¹, TANG Min², ZHANG Mingjun³, TANG Xiaojun¹

1. Department of Epidemiology, College of Public Health and Management, Chongqing Medical University, Chongqing 400016, China;
2. College of Medical Examination, Chongqing Medical University, Chongqing 400016, China;
3. Clinical Laboratory, Chongqing Jiulongpo First People's Hospital, Chongqing 400050, China

Received:2014-09-29 Published:2015-08-01

摘要/Abstract

摘要：

目的: 探讨男性人群中载脂蛋白E(APOE)的基因多态性与血脂水平的关联性,为APOE基因作为诊断血脂异常指标提供理论依据。方法: 从3家重庆医院体检中心随机抽取男性356人,其中242例高血脂患者作为病例组,114名健康者作为对照组。检测研究对象静脉血中总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL-C)和低密度脂蛋白(LDL-C)水平,并测量其身高和体质量。采用基因芯片测序方法对APOE基因进行分型。结果: APOE基因型在人群中分布,共检测出6种基因型,其中E3/3频数最高,为69.10%;APOE等位基因中E2频数为17.98%,E3频数为75.84%,E4频数为6.18%。在病例组中,不同APOE基因型高血脂患者TC水平比较差异有统计学意义(P<0.05),且APOE4基因携带者的TC水平明显高于APOE2和APOE3基因携带者(P<0.05)。多元线性回归分析,APOE多态性与TC、TG呈正相关关系(r=0.157,P<0.05;r=0.276,P<0.05),与HDL水平呈负相关关系(r=-0.153,P<0.01)。结论: 中国男性人群中APOE基因多态性对血脂水平有一定的影响,APOE4基因型携带者TC和TG水平有升高的趋势。

关键词: 载脂蛋白E, 基因型, 体质量指数, 血脂

Abstract:

Objective To investigate the relationships between apolipoprotein E(APOE) genotypes and blood lipid levels in the male population,and to provide theoretical basis for the diagnosis of dyslipidemia indexes. Methods A cross-sectional health survey was carried out in three hospitals in Chongqing.The random samples came from 365 males in physical examination centers;among them 242 patients with high level of blood lipids were used as case group;and the other 114 healthy subjects were used as control group.The plasma levels of total cholesterol (TC),triglycerides(TG),high-density lipoprotein cholesterol (HDL-C),low-density lipoprotein cholesterol(LDL-C) were determined;the heights and body weights were detected.The genotypes of APOE were evaluated by gene chip sequencing method. Results Six kinds of genotypes in the population were find,among them E3/3 had the highest frequency of APOE genotypes (69.10%),meanwhile the frequency of APOE E2 allele was 17.93%.The frequency of E3 was 75.84%,and the frequency of E4 was 6.18%.There were statistically significant differences of the TC levels between the patients with different APOE genotypes in case group (P<0.05),meanwhile the E4 carriers had a higher TC level than E2 and E3 carriers (P<0.05).The multiple linear regression analysis showed that APOE polymorphism was positively related with the levels of TC and TG(r=0.157,P<0.05;r=0.276,P<0.05),however it was negatively related with the HDL level(r=-0.153,P<0.05). Conclusion The polymorphism of APOE is related to the blood lipid levels in Chinese male population,while the patients with APOE4 genotype have higher TC and TG levels.

Key words: apolipoprotein E, genotype, body mass index, blood lipid

中图分类号:

R589.2

傅倩晰, 周莉, 唐敏, 张名均, 唐晓君. 男性人群中载脂蛋白E基因多态性与血脂水平的关联性分析[J]. 吉林大学学报(医学版), 2015, 41(04): 808-813.

FU Qianxi, ZHOU Li, TANG Min, ZHANG Mingjun, TANG Xiaojun. Analysis on association between polymorphism of apolipoprotein E genotypes and blood lipid levels in male population[J]. Journal of Jilin University Medicine Edition, 2015, 41(04): 808-813.

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男性人群中载脂蛋白E基因多态性与血脂水平的关联性分析

Analysis on association between polymorphism of apolipoprotein E genotypes and blood lipid levels in male population

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