MMP-2和MMP-9在食管鳞状细胞癌患者癌组织中的表达及其意义

doi:10.13481/j.1671-587x.20150428

吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (04): 814-819.doi: 10.13481/j.1671-587x.20150428

MMP-2和MMP-9在食管鳞状细胞癌患者癌组织中的表达及其意义

孙晓宏¹, 房福元², 李卉³, 尹娜³, 庞作良¹, 李惠武³

1. 新疆医科大学附属肿瘤医院胸外科, 新疆乌鲁木齐 830011;
2. 广东省深圳市人民医院胸外科, 广东深圳 518020;
3. 新疆医科大学基础医学院科研中心, 新疆乌鲁木齐 830001

收稿日期:2014-12-02 发布日期:2015-08-01
通讯作者: 李惠武,教授,博士研究生导师(Tel:0991-4366434,E-mail:huiwuli1234@163.com) E-mail:huiwuli1234@163.com
作者简介:孙晓宏(1974-),男,山东省金乡县人,讲师,医学博士,主要从事胸部肿瘤外科诊治方面的研究。
基金资助:
国家自然科学基金资助课题(81350021)

Expressions of MMP-2 and MMP-9 in tumor tissue of patients with esophageal squamous cell carcinoma and their significances

SUN Xiaohong¹, FANG Fuyuan², LI Hui³, YIN Na³, PANG Zuoliang¹, LI Huiwu³

1. Department of Thoracic Surgery, Affiliated Tumor Hospital, Xinjiang Medical University, Urumqi 830011, China;
2. Department of Thoracic Surgery, People's Hospital, Shenzhen City, Guangdong Province, Shenzhen 518020, China;
3. Research Center, School of Basic Medical Sciences, Xinjiang Medical University, Urumqi 830001, China

Received:2014-12-02 Published:2015-08-01

摘要/Abstract

摘要：

目的: 探讨食管鳞状细胞癌患者癌组织中基质金属蛋白酶2(MMP-2)和基质金属蛋白酶9(MMP-9)表达,分析二者的关联性。方法: 选取食管鳞状细胞癌手术离体标本100例,取癌组织和对应的癌旁正常组织,采用逆转录-聚合酶链式反应(RT-PCR)法检测MMP-2和MMP-9 mRNA表达,采用Western blotting法检测MMP-2和MMP-9蛋白表达;采用Pearson相关分析法分析癌组织中MMP-2和MMP-9 mRNA表达的相关性。结果: 食管鳞状细胞癌组织和癌旁正常组织中MMP-2 mRNA阳性表达率分别为89.0%(89/100)和76.0%(76/100),两者比较差异有统计学意义(χ²=5.853,P=0.016);食管鳞状细胞癌组织和癌旁正常组织中MMP-9 mRNA阳性表达率分别为48.0%(48/100)和16.0%(16/100),两者比较差异有统计学意义(χ²=23.529,P=0.000)。MMP-2 mRNA阳性表达率与食管鳞状细胞癌的浸润深度有关联(χ²=4.971,P=0.026),与淋巴结转移无关联(χ²=2.127,P=0.145);MMP-9 mRNA阳性表达率与食管鳞状细胞癌的浸润深度有关联(χ²＝4.421,P=0.035),与淋巴结转移无关联(χ²=0.617,P=0.432)。MMP-2 mRNA与MMP-9 mRNA在食管鳞状细胞癌组织中的阳性表达率呈正相关关系(r=0.274,P=0.006)。结论: MMP-2和MMP-9可能参与了食管鳞状细胞癌的发生发展,但在食管鳞状细胞癌淋巴结转移方面可能不发挥关键作用。

关键词: 食管肿瘤, 癌, 鳞状细胞, 基质金属蛋白酶2, 基质金属蛋白酶9, 逆转录-聚合酶链式反应

Abstract:

Objective To explore the expressions of matrix metalloproteinase-2(MMP-2) and matrix metalloproteinase-9(MMP-9) in the tumor tissue of the patients with esophageal squamous cell carcinoma, and to analyze the correlation between the expressions of MMP-2 and MMP-9. Methods 100 spicemens of surgical removed esophageal squamous cell carcinoma were chosen. The tumor tissue and the adjacent normal esophagel mucosa tissue were selected.RT-PCR method was used to detect the expressions of MMP-2 and MMP-9 mRNA in the esophageal squamous cell carcinoma tissue and the adjacent normal tissue.Western blotting method was used to testify the expressions of MMP-2 and MMP-9 proteins;the correlation between the expressions of MMP-2 and MMP-9 was analyzed by Pearson correlation analysis. Results The positive expression rate of MMP-2 mRNA in carcinoma tissue was 89.0%(89/100), the positive expression rate of MMP-2 mRNA in adjacent normal tissue was 76.0%(76/100),and there was statistical difference between two groups(χ²=5.853,P=0.016).The positive expression rate of MMP-9 mRNA in carcinoma tissue was 48.0%(48/100),the positive expression rate in adjacent normal tissue was 16.0%(16/100),and there was statistical difference of the positive expression rate between two groups(χ²=23.529,P=0.000).There was a positive correlation between the expression of MMP-2 mRNA and the tumor invasion depth(χ²=4.971,P=0.026), but there was no correlation between the expression of MMP-2 mRNA and the lymph node metastasis(χ²=2.127,P=0.145).There was a positive correlation between the expression of MMP-9 mRNA and the tumor invasion depth(χ²=4.421,P=0.035), but there was no correlation between the expression of MMP-9 mRNA and lymph node metastasis(χ²=0.617,P=0.432). There was positive correlationship between the positive expression rates of MMP-2 mRNA and MMP-9 mRNA in esophageal cancer tissue(r=0.274,P=0.006). Conclusion The MMP-2 and MMP-9 may play a very important role in the occurrence and development of esophageal squamous cell carcinoma,however they have no affection on lymph node metastasis of esophageal squamous cell carcinoma.

Key words: esophageal neoplasms, carcinoma,squamous cell, matrix metalloproteinase-2, matrix metalloproteinase-9, reverse transcription-polymerase chain reaction

中图分类号:

R735.1

孙晓宏, 房福元, 李卉, 尹娜, 庞作良, 李惠武. MMP-2和MMP-9在食管鳞状细胞癌患者癌组织中的表达及其意义[J]. 吉林大学学报(医学版), 2015, 41(04): 814-819.

SUN Xiaohong, FANG Fuyuan, LI Hui, YIN Na, PANG Zuoliang, LI Huiwu. Expressions of MMP-2 and MMP-9 in tumor tissue of patients with esophageal squamous cell carcinoma and their significances[J]. Journal of Jilin University Medicine Edition, 2015, 41(04): 814-819.

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MMP-2和MMP-9在食管鳞状细胞癌患者癌组织中的表达及其意义

Expressions of MMP-2 and MMP-9 in tumor tissue of patients with esophageal squamous cell carcinoma and their significances

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