关键词: 多酸化合物, 洗手液, 甲型流感病毒, 毒性

Abstract:

Objective To prepare a new hand-washing agent with heterpolyoxometalates and to study its anti-A-influenza virus effect and toxicity.
Methods The anti-virus effects of the two compounds K10Na［(VO)3(SbW9O33)2］?26H2O(PM-1001) and K15［Pr(BW11O39)2］?28H2O(Pr-B)　were detected on MDCK cells.Three levels  of heterpolyoxometalates(high,middle,and low doses)were chosen to add into hand-washing agents; the anti-A-influenza virus effect
 
test was performed according to the American Society for Testing and Materials(ASTM) standard assessment protocols.In this test,10 volunteers were randomly divided into two groups for testing hand-washing agents with PM-1001 and Pr-B respectively.Every volunteer’s ten fingers were divided into 5 groups randomly.After all the finger taps were contaminated with 0.02　mL of 107.5 tissue culture infectious dose (TCID50)/0.1　mL live human influenza A virus, the H1N1 titers  were assessed with MDCK cell culture method after  contaminating,air drying and treatment with different hand-washing agents.The
concentrations and  viabilities of H1N1 on hands  30 and 60 min after hand drying  were also assessed.The hand-washing agent with  high dose of PM-1001 was chosen for toxicity assessment using Kunming mice and rabbits according to Disinfection Technical Specifications (2008).
Results The  therapeutic indexes  (TI) of PM-1001 and Pr-B   were  82.68 and 69.13.The H1N1 concentration in  control group was 104.33±0.66TCID50/0.1　mL
.The fingers in treatment groups had no viable H1N1 recovered.The virus concentrations in  treatment groups had significant differences compared with  control group(P<0.001).The H1N1 virus concentrations  in  30 and 60 min groups were 103.98 ±0.42TCID50/0.1　mL and 103.83±0.35TCID50/0.1 mL and had no significant differences compared with  control group(P>0.05).The LD50 in  mouse acute toxicity test  was >5 000 mg/kg^-1,the rabbit skin irritation index was 0.33,and the eye stimulation indexes of three rabbits were 0,0.67, and 0.33. Conclusion The new  hand-washing agents including PM-1001 and Pr-B are highly effective in reducing influenza A virus on human hands.It has no toxicity and no stimulation to skin and eyes of rabbits.
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Key words: heterpolyoxometalates, hand-washing agent, influenza A virus, toxicity