关键词: 偏头痛, 正清风痛宁, 肿瘤坏子因子&alpha, 白细胞介素1&beta, 炎性细胞

Abstract:

Objective To observe the changes of the expressions of tumor necrosis factor alpha (TNF-α) and interleukin-1 beta (IL-1β) in the brainstem and the number of inflammatory cells in brainstem subarachnoid of the rat models with migraine, and to explore the inhibitory effect of Zhengqingfengtongning on aseptic neurogenic inflammation(ANI) of the rats with migraine. Methods 60 healthy Wistar rats were randomly divided into blank control group, model group, sumatriptan(6 mg·kg^-1·d^-1) group, low dose of Zhengqingfengtongning(100 mg·kg^-1·d^-1) group, middle dose of Zhengqingfengtongning(150 mg·kg^-1·d^-1) group and high dose of Zhengqingfengtongning(200 mg·kg^-1·d^-1)group(n=10).The migraine rat model was established by subcutaneous administration of nitroglycerin.The brain and mater of the rats were decapitated 4 h after medical intervention.The expressions of TNF-α and IL-1β in the rat brainstem were evaluated by immunohistochemistry, and the number of TNF-α and IL-1β positive cells under microscope was counted.HE staining was used to observe the changes of the number of inflammatory cells in the brainstem subarachnoid of the rats and the number of inflammatory cells was counted under light microscope of per unit area subarachnoid space(in a view for unit). Results Compared with blank control group, the number of TNF-α and IL-1β expression positive cells in the brainstem of the rats in model group was increased significantly, and the differences were statistically significant(P<0.01);compared with model group, the number of TNF-α and IL-1β expression positive cells in the brainstem of the rats in middle and high doses of Zhengqingfengtongning groups and sumatriptan group was significantly decreased, the differences were statistically significant(P<0.05).Compared with blank control group, the number of inflammatory cells(mainly lymphocytes and monocytes) in the subarachnoid space of the rats in model group was significantly increased(P<0.01);compared with models group, the number of inflammatory cells in the subarachnoid space in different doses of Zhengqingfengtongning groups and sumatriptan group were significantly reduced(P<0.01). Conclusion Zhengqingfengtongning may inhibit the ANI of the rat model with migraine, suggesting that Zhengqingfengtongning has the effect of anti-inflammatory and analgesia in treatment of migraine.

Key words: migraine, Zhengqingfengtongning, tumor necrosis factor α, interleukin-1β, inflammatory cells