关键词: 人参皂苷Re, 紫外线, 流式细胞术, 细胞凋亡, 超氧化物歧化酶

Abstract: Abstract:Objective To investigate the protective effects of ginsenoside Re  on human embryonic fibroblast RSa cells damaged by ultraviolet C(UVC),and to discuss the anti-UVC me
chanism of ginsenoside Re.Methods The RSa cells were irradiated with different dosages of UVC,and the RSa cells were treated with  50 mg?L-1 ginsenosides   before and after  irradiation(UVC+Re group),at the same time,  UVC model group and UVC+Rg1 group were established. MTT assay was used to analyze the  survival rate of cells;apoptosis and cell cycle were measured by flow cytometry (FCM) method,and the activity of superoxide dismutase (SOD) was determined by enzyme biochemical methods.The survival rate of cells after treated with SOD inhibitor was detected by colony formation assay. Results The results of MTT assay showed that the survival rate of  the cells in  UVC+ Re group and UVC+ Rg1 group were significantly increased   compared with UVC model group(P<0.01 or P<0.05). The FCM results showed that  different dosages of UVC could induce early apoptosis of the RSa cells. The apoptotic  rate of cells in UVC+Re group was significantly decreased compared  with UVC model group. The cell cycle detection results showed that the  percentage of the RSa cells at G2 phase was significantly increased after UVC irradiation（P<0.01）,and the percentage  of the Rsa cells at G2 phase  in UVC+ Re group was significantly decreased   compared with UVC model group（P<0.01),and the ratio of the RSa cells at G2 phase in UVC+Re group was significantly decreased.Compared with UVC model group,the activity of SOD in UVC+ Re group was incresed （P<0.01）. Conclusion Ginsenoside Re has anti-UVC efficiency and can enhance the survival rate of RSa cells,and inhibit early   apoptosis and increase the SOD activity.

Key words: ginsenoside Re, ultraviolet C, flow cytometry, apoptosis, superoxide dismutase