关键词: 宫颈肿瘤, 免疫组织化学, 聚ADP核糖聚合酶-1, 半胱氨酸天冬氨酸蛋白酶, 存活
素, Bax

Abstract: Objective To explore the expressions of poly ADP-ribose polymerase(PARP-1),cysteinyl aspartate specific proteinase(Caspase-3),survivin and Bax in cervical squamous cell carcinoma tissues and their relationships with the  clinicopathogical characteristics of cervical carcinoma,and to provide experimental basis for early diagnosis and  treatment for cervical carcinoma.Methods Immunohistochemical method was used to detect the expression rates of PARP-1,Caspase-3,survivin and Bax in specimens of 60 cases of cervical squamous cell carcinoma,40 cases of cervical carcinoma in situ and 20 cases of  normal cervical epithelium (NCE) adjacent to carcinoma.The relationships between their expression levels and clinical stages,pathological types and lymph node metastasis or not were analyzed.Results The positive expression rates of PARP-1 in cervical squamous cell carcinoma,cervical carcinoma in situ and NCE tissue were 68.33%,62.50%,and 25.00%,respectively;the positive expression rates of Caspase-3 in cervical squamous cell carcinoma,cervical carcinoma in situ and NCE tissue were 43.33%,47.50%,and 70.00%, respectively;the positive expression rates of survivin  were 95.00%,92.50%,and 5.00%,respectively;the positive expression rates of Bax  were 31.67%,32.50%,and 75.00%,respectively.The positive expression rates of PARP-1 and survivin    in  squamous cell carcinoma and carcinoma in situ groups were lower than those in NCE group (P<0.001);the positive expression rates of Caspase-3 and Bax  in  squamous cell carcinoma and carcinoma in situ groups were higher than those in NCE group (P<0.01).The  positive expression rates  of  PARP-1,survivin and Bax had relationships  with the clinical stage,histological type,and lymph node metastasis(P<0.01);but the positive expression rate of  Caspase-3 didn’t have relationship with those indexes mentioned above(P>0.01).The  positive expression rates of PARP-1 and Caspase-3 in cervical squamous cell carcinoma tissue were negatively correlated （r=－0.71,P=0.043）;the positive  expression rates of  survivin and Bax in cervical squamous cell carcinoma tissue  were negatively correlated（r=－0.63,P=0.038）.Conclusion The up-regulation  of the expression levels  of PARP-1 and survivin and the down-regulation of the levels of Caspase-3 and Bax expression are likely to play an important role in the invasion and metastasis of cervical squamous cell carcinoma.

Key words: cervical neoplasms, immunohistochemistry, poly ADP-ribose polymerase, cysteinyl aspartate specific proteinase, survivin, Bax