吉林大学学报(医学版) ›› 2017, Vol. 43 ›› Issue (02): 353-355.doi: 10.13481/j.1671-587x.20170227

• 临床研究 • 上一篇    下一篇

p16INK4a蛋白在蒙古族患者宫颈病变组织中的表达及其意义

强欣1, 霍文艳1,2, 杨灿1, 范亚楠1, 石彩云1, 白海花1,2   

  1. 1. 内蒙古民族大学人类基因组与遗传病研究所, 内蒙古 通辽 028000;
    2. 内蒙古民族大学附属医院分子生物学实验室, 内蒙古 通辽 028007
  • 收稿日期:2016-05-04 出版日期:2017-03-28 发布日期:2017-03-31
  • 通讯作者: 白海花,教授,硕士研究生导师(Tel:0475-8232796,E-mail:hh_bai@163.com) E-mail:hh_bai@163.com
  • 作者简介:强欣(1968-),女,内蒙古自治区通辽市人,副教授,主要从事宫颈癌病理学方面的研究。
  • 基金资助:
    内蒙古自治区科技厅科研基金资助课题(201502103)

Expressions of p16INK4a protein in cervical lesion tissues of Mongolian patients and their significances

QIANG Xin1, HUO Wenyan1,2, YANG Can1, FAN Yanan1, SHI Caiyun1, BAI Haihua1,2   

  1. 1. Institute of Human Genome and Genetic Diseases, Inner Mongolia University for Nationalities, Tongliao 028000, China;
    2. Molecular Biology Laboratory, Affiliated Hospital, Inner Mongolia University for Nationalities, Tongliao 028000, China
  • Received:2016-05-04 Online:2017-03-28 Published:2017-03-31

摘要: 目的:检测p16INK4a蛋白在蒙古族患者宫颈病变组织中的表达,探讨其与蒙古族患者宫颈癌发生发展的关系。方法:选取100例蒙古族患者宫颈癌、宫颈上皮内瘤变(CIN)、慢性宫颈炎和子宫平滑肌瘤病变组织石蜡切片,分为宫颈癌组25例、CIN组35例、慢性宫颈炎组20例和子宫平滑肌瘤组20例,利用免疫组织化学SP法检测各组石蜡切片组织中p16INK4a蛋白表达。结果:蒙古族患者宫颈癌、CIN、慢性宫颈炎、子宫平滑肌瘤组织中p16INK4a蛋白阳性表达率分别为100.0%、74.3%、25.0%和10.0%。多个样本比较的K-W H秩和检验,宫颈癌组p16INK4a蛋白阳性表达率高于CIN组、慢性宫颈炎组和子宫平滑肌瘤组(P<0.05)。结论:p16INK4a蛋白可以作为筛选蒙古族早期宫颈癌患者的一种检测指标。

关键词: p16INK4a蛋白, 宫颈肿瘤, 宫颈上皮内瘤变, 慢性宫颈炎, 子宫平滑肌瘤, INK4a基因

Abstract: Objective: To detect the expressions of p16INK4a protein in the cervical lesion tissues of the Mongolian patients, and to explore the relationship between its expression and the occurrence and development of cervical cancer in the Mongolian patients. Methods: A total of 100 cases of paraffin sections of cervical cancer, cervical intraepithelial neoplasia(CIN),chronic cervicitis and uterine leiomyoma were divided into 25 cases of cervical cancer, 35 cases of CIN, 20 cases of chronic cervicitis, and 20 cases of uterine leiomyoma groups. The expressions of p16INK4a protein in different cervical tissues were detected by immunohistochemical SP method. Results: The positive rates of p16INK4a protern in cervical cancer, CIN, chronic cervicitis and uterine leiomyoma tissnes were 100.0%, 74.3%, 25.0%,and 10.0%, respectively.The results of K-WH rank sum test for multiple sample comparisons showed that the positive expression rate of p16INK4a protein in cervical cancer tissue was significantly higher than those in CIN, chronic cervicitis and uterine leiomyoma tissues(P<0.05). Conclusion: p16INK4a protein can be used as a indicator to screen the Mongolian patients with early cervical cancer.

Key words: INK4a gene, p16INK4a, cervical intraepithelial neoplasia, chronic cervicitis, cervical neoplasms, uterine leiomyoma

中图分类号: 

  • R737.33