MNU法诱导大鼠原位膀胱癌模型的建立及MRI的诊断价值

doi:10.13481/j.1671-587x.20160410

吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (04): 685-689.doi: 10.13481/j.1671-587x.20160410

MNU法诱导大鼠原位膀胱癌模型的建立及MRI的诊断价值

宋志强^1,2, 沈海山², 沈俊^1,2, 吴建臣², 张明³, 李然伟³, 张继珍², 李胜文^1,2

1. 清华大学医学中心, 北京 100084;
2. 清华大学第一附属医院泌尿外科, 北京 100016;
3. 吉林大学第二医院泌尿外科, 吉林长春 130041

收稿日期:2015-12-12 发布日期:2016-07-20
通讯作者: 李胜文,主任医师,教授,博士研究生导师(Tel:010-64308522,E-mail:swli@mails.tsinghua.edu.cn) E-mail:swli@mails.tsinghua.edu.cn
作者简介:宋志强(1984-),男,吉林省汪清县人,在读医学博士,主要从事泌尿系统肿瘤诊断与治疗方面的研究。
基金资助:
国家自然科学基金青年科学基金资助课题（81201527）；清华-周大福医学研究专项基金资助课题（202836019-04）；清华-裕元医学科学研究基金资助课题（20240000538）

Establishment of rat model of orthotopic bladder cancer induced by MNU and diagnosis value of MRI

SONG Zhiqiang^1,2, SHEN Haishan², SHEN Jun^1,2, WU Jianchen², ZHANG Ming³, LI Ranwei³, ZHANG Jizhen², LI Shengwen^1,2

1. Medical Center, Tsinghua University, Beijing 100084, China;
2. Department of Urology, First Hospital of Tsinghua University, Beijing 100016, China;
3. Department of Urology, Second Hospital, Jilin University, Changchun 130041, China

Received:2015-12-12 Published:2016-07-20

摘要/Abstract

摘要：

目的：构建N-甲基亚硝基脲（MNU）诱导的大鼠原位膀胱癌模型，探讨利用磁共振成像技术（MRI）对膀胱癌模型进行无创诊断的价值。方法：60只SD雌性大鼠分为实验组45只和对照组15只。实验组大鼠定期膀胱灌注MNU，每次2mg，每2周1次，共4次。对照组大鼠同时膀胱灌注生理盐水，每次0.2mL。于第14周末麻醉大鼠后进行MRI扫描检测，检测后处死大鼠，取膀胱组织进行病理学检测。结果：实验组45只大鼠14周末存活43只，死亡2只，存活率95.6%，死亡率4.4%；经MRI扫描膀胱均见异常信号，提示肿瘤形成，与病理检查结果一致，存活大鼠成瘤率为100%。对照组15只大鼠14周末存活14只，死亡1只，存活率93.3%，死亡率6.7%；经MRI检查未发现膀胱肿瘤，病理检查未见膀胱癌，成瘤率为0。2组大鼠成瘤率比较差异有统计学意义（P < 0.05），死亡率比较差异无统计学意义（P > 0.05）。结论：MNU膀胱灌注诱导大鼠原位膀胱癌模型方法简便、可靠；MRI扫描结果与病理学检测结果一致，可作为该模型的无创鉴定方法。

关键词: 疾病模型, 动物, 膀胱肿瘤, N-甲基亚硝基脲, 磁共振成像, 活体鉴定

Abstract:

Objective: To construct the rat models of orthotopic bladder cancer induced by N-methyl-nitrosourea(MNU),and to evaluate the value of magnetic resonance imaging(MRI) in the noninvasive diagnosis of the bladder cancer model. Methods: Sixty femail SD rats were divided into experiment group (n=45) and control group (n=15). The rats experiment group were induced with MNU (2 mg per rat) by intravesical administration every other week,for 4 times. Meantime,the rats in control group were treated with normal saline (0.2 mL per rat) by intravesical administration. At the end of the 14th week,all rats were examined by MRI and the pathological changes of bladder tissue were detected. Results: In experiment group,43 rats were alived and 2 rats were died at the end of the 14th week;the survial rate was 95.6% and the death rate was 4.4%;the abnormal signals were found in each of 43 rats by MRI which manifested as bladder tumor,and the same results were identified by pathology;the tumor formation rate was 100%. In control group,14 rats were alived and 1 rat was died at the end of the 14th week;the survival rate was 93.3%,and the death rate was 6.7%;there was no abnormal signal in the MRI examination and no bladder cancer in the pathological examination; the tumor formation rate was 0. The tumor formation rates of bladder cancer of the rats in two groups had significant difference (P < 0.05),and the death rate had no significant difference (P > 0.05). Conclusion: The method to establish the rat models of orthotopic bladder cancer induced by MNU is simple and reliable;the results of MRI are consistent with the pathological results and MRI examination is a reliable diagnostic method concerning this model.

Key words: disease model, animals, bladder neoplasms, N-methyl-nitrosourea, magnetic resonance imaging, identification of living body

中图分类号:

R737.14

宋志强, 沈海山, 沈俊, 吴建臣, 张明, 李然伟, 张继珍, 李胜文. MNU法诱导大鼠原位膀胱癌模型的建立及MRI的诊断价值[J]. 吉林大学学报(医学版), 2016, 42(04): 685-689.

SONG Zhiqiang, SHEN Haishan, SHEN Jun, WU Jianchen, ZHANG Ming, LI Ranwei, ZHANG Jizhen, LI Shengwen. Establishment of rat model of orthotopic bladder cancer induced by MNU and diagnosis value of MRI[J]. Journal of Jilin University Medicine Edition, 2016, 42(04): 685-689.

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MNU法诱导大鼠原位膀胱癌模型的建立及MRI的诊断价值

Establishment of rat model of orthotopic bladder cancer induced by MNU and diagnosis value of MRI

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