MT01对牙龈卟啉单胞菌感染成骨细胞MG63中Ⅰ型胶原mRNA表达的促进作用及其意义

doi:10.13481/j.1671-587x.20160409

吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (04): 681-684.doi: 10.13481/j.1671-587x.20160409

MT01对牙龈卟啉单胞菌感染成骨细胞MG63中Ⅰ型胶原mRNA表达的促进作用及其意义

刘引^1,2, 申玉芹¹, 高涵^1,2, 费鸿博^1,2, 胡天琦^1,2, 李洋洋^1,2, 顾中一^1,2, 林崇韬¹

1. 吉林大学口腔医院牙周病科, 吉林长春 130021;
2. 吉林大学口腔医院吉林省牙发育及颌骨重塑与再生重点实验室, 吉林长春 130021

收稿日期:2015-12-25 发布日期:2016-07-20
通讯作者: 林崇韬,教授,硕士研究生导师(Tel:0431-88796039,E-mail:linct@jlu.edu.cn) E-mail:linct@jlu.edu.cn
作者简介:刘引(1990-),女,安徽省宿州市人,在读医学硕士,主要从事牙周病病因及组织再生机制方面的研究。
基金资助:
国家自然科学基金面上项目资助课题（81371153）；吉林省科技厅自然科学基金面上项目资助课题（20150101173JC）

Effects of MT01 on expression of collagen Ⅰ mRNA in osteoblasts MG63 infected by Porphyromonas gingivalis and its significance

LIU Yin^1,2, SHEN Yuqin¹, GAO Han^1,2, FEI Hongbo^1,2, HU Tianqi^1,2, LI Yangyang^1,2, GU Zhongyi^1,2, LIN Chongtao¹

1. Department of Periodontology, Stomatology Hospital, Jilin University, Changchun 130021, China;
2. Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Jilin University, Changchun 130021, China

Received:2015-12-25 Published:2016-07-20

摘要/Abstract

摘要：

目的：检测单链寡脱氧核苷酸MT01 作用下牙龈卟啉单胞菌（Pg）感染成骨细胞MG63中Ⅰ型胶原（ColⅠ）mRNA 表达水平，探讨MT01对感染状态下成骨细胞成骨向分化的影响。方法：体外培养的MG63细胞分为空白对照组、MT01组、Pg组和MT01+Pg组。按分组情况进行相应处理，加入MT01（质量浓度1 mg·L^-1），以1 mg·L^-1PBS作对照共孵育3h后，以100∶1的感染复数（MOI）加入Pg悬液共培养。4组细胞分别孵育2、4、6、8、12和24h后采用RT-PCR法检测MG63细胞中ColⅠ mRNA表达水平。结果：与空白对照组比较，MT01和MT01+Pg组MG63细胞中ColⅠ mRNA表达水平在2和4h时无明显变化（P > 0.05），8、12和24h时MT01组细胞中ColⅠ mRNA表达水平明显升高（P < 0.05或P < 0.01）；与Pg组比较，2和4h时MT01+Pg组细胞中ColⅠ mRNA呈低表达，6、8、12和24h时ColⅠ mRNA表达水平升高（P < 0.05或P < 0.01）。结论：MT01上调感染状态下MG63细胞中ColⅠ mRNA的表达水平，MT01可促进感染状态下成骨细胞成骨向分化。

关键词: 单链寡脱氧核苷酸MT01, 牙龈卟啉单胞菌, 人成骨细胞, Ⅰ型胶原

Abstract:

Objective: To investigate the effect of MT01 on the differentiation of osteoblasts under infected condition through determining the expression level of collagen Ⅰ(ColⅠ) mRNA in MG63 cells treated with Porphyromonas gingivalis(Pg). Methods: The cultured MG63 cells were divided into blank control,MT01,Pg, and MT01+Pg groups.MT01 at a concentration of 1 mg·L^-1 was added into the MG63 cells,and the cells were incubated for 3 h.The cells treated with PBS (1 mg·L^-1) were used as control group.Then Pg(MOI=100∶1)was added.Real-time PCR was used to detect the expression levels of ColⅠ mRNA in MG63 cells at 2,4,6,8,12 and 24 h after incubation. Results: Compared with blank control group,the levles of ColⅠ mRNA in the MG63 cells in MT01 and MT01+Pg groups had no significant changes at 2 and 4 h(P > 0.05);the ColⅠ mRNA expression levels in MT01 group at 8,12 and 24 h were increased(P < 0.05 or P < 0.01).Compared with Pg group, the expression levels of ColⅠ mRNA in MT01+ Pg at 2 and 4 h were decreased,but the expression levels of ColⅠ mRNA were increased at 6,8,12 and 24 h(P < 0.05 or P < 0.01). Conclusion: MT01 can up-regulate the expression level of ColⅠ mRNA in the infected MG63 cells;MT01 could promot the differentiation of osteoblasts under infected condition.

Key words: specific sequence oligodeoxynucleotide MT01, Porphyromonas gingivalis, human osteoblast cell, collagen Ⅰ

中图分类号:

Q254

刘引, 申玉芹, 高涵, 费鸿博, 胡天琦, 李洋洋, 顾中一, 林崇韬. MT01对牙龈卟啉单胞菌感染成骨细胞MG63中Ⅰ型胶原mRNA表达的促进作用及其意义[J]. 吉林大学学报(医学版), 2016, 42(04): 681-684.

LIU Yin, SHEN Yuqin, GAO Han, FEI Hongbo, HU Tianqi, LI Yangyang, GU Zhongyi, LIN Chongtao. Effects of MT01 on expression of collagen Ⅰ mRNA in osteoblasts MG63 infected by Porphyromonas gingivalis and its significance[J]. Journal of Jilin University Medicine Edition, 2016, 42(04): 681-684.

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MT01对牙龈卟啉单胞菌感染成骨细胞MG63中Ⅰ型胶原mRNA表达的促进作用及其意义

Effects of MT01 on expression of collagen Ⅰ mRNA in osteoblasts MG63 infected by Porphyromonas gingivalis and its significance

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