复方当归注射液对缺血性脑损伤大鼠BDNF表达的影响

doi:10.13481/j.1671-587x.20150601

吉林大学学报(医学版) ›› 2015, Vol. 41 ›› Issue (06): 1103-1106.doi: 10.13481/j.1671-587x.20150601

• 基础研究 • 下一篇

复方当归注射液对缺血性脑损伤大鼠BDNF表达的影响

汤轶波, 袁伟畅, 朱春燕, 张赛, 张玮, 张林朋, 南一楠, 牟天龙, 王旭, 杨晓敏, 何雪飞, 潘彦舒

北京中医药大学基础医学院病理学系, 北京 100029

收稿日期:2015-08-21 出版日期:2015-11-28
通讯作者: 潘彦舒,教授,硕士研究生导师(Tel:010-64286887,E-mail:panyanshu@263.net) E-mail:panyanshu@263.net
作者简介:汤轶波(1980-),女,江苏省张家港市人,讲师,医学博士,主要从事中医药治疗缺血性脑病方面的研究。
基金资助:
国家自然科学基金资助课题(81271897);国家重点基础研究发展计划(973计划)资助课题(2012CB518602);北京中医药大学自主项目资助课题(2013-JYBZZ-JS-120)

Effect of Compound Angelica Injection on expression of brain derived neurotrophic factor of rats with cerebral ischemia injury

TANG Yibo, YUAN Weichang, ZHU Chunyan, ZHANG Sai, ZHANG Wei, ZHANG Linpeng, NAN Yinan, MU Tianlong, WANG Xu, YANG Xiaomin, HE Xuefei, PAN Yanshu

Department of Pathology, College of Basic Medical Science, Beijing University of Chinese Medicine, Beijing 100029, China

Received:2015-08-21 Online:2015-11-28

摘要/Abstract

摘要：

目的:观察复方当归注射液(CAI)对局灶性脑缺血损伤模型大鼠患侧脑组织及血清中脑源性神经营养因子(BDNF)表达水平的影响,探讨CAI对脑缺血损伤的作用机制。方法:50只SD大鼠随机分为假手术组(10只)和局灶性脑缺血损伤组(40只)。局灶性脑缺血损伤组采用线栓法复制大鼠局灶性脑缺血损伤模型,将造模成功的30只大鼠随机分为模型组、CAI组和阳性对照依达拉奉组,每组10只。造模大鼠苏醒后进行神经功能评分,7 d后取材,HE染色检测大鼠脑组织的病理学改变,测定大鼠血清及缺血侧脑组织中BDNF的表达水平。结果:与假手术组比较,模型组大鼠神经功能评分增加(P<0.05),大鼠神经细胞出现肿胀、坏死和细胞间质水肿等改变,缺血侧脑组织及血清中BDNF表达水平有一定的上升趋势;CAI组大鼠缺血侧脑组织BDNF表达水平显著升高(P<0.05),血清BDNF表达水平有所升高。与模型组比较,CAI组大鼠神经功能评分降低,大鼠神经细胞轻微水肿,缺血侧脑组织及血清BDNF表达水平有所升高。结论:CAI可能具有提高脑内BDNF表达的作用,其作用机制可能与机体在缺血损伤情况下脑内相应的神经因子做出应激反应有关。

关键词: 复方当归注射液, 局灶性脑缺血, 脑源性神经营养因子, 疾病模型,动物

Abstract:

Objective To investigate the effect of Compound Angelica Injection (CAI) on the expressions of brain derived neurotrophic factor (BDNF) in brain tissue and serum in the rats with cerebral ischemia injury, and to explore the related mechanism of effect of CAI on cerebral ischemia injury. Methods 50 SD rats were randomly divided into sham operation group (n=10) and cerebral ischemia injury group (n=40), and the rats in cerebral ischemia injury group were reproduced by middle cerebral artery occlusion (MCAO). Thirty successfully modeling rats were randomly divided into model group, CAI group and edaravone group (n=10). The neurologic scores of the rats after waking up were measured. The pathomorphology of brain tissue of the rats was detected by HE staining 7 d later. The levels of BDNF in the brain tissue and serum of the rats were detected. Results Compared with sham operation group, the neurologic score of the rats in model group was increased (P<0.05); the neurocytes of rats were changed to swell, necrosis, and interstitial edema; the levels of BDNF in brain tissue and serum showed a tendency of increasing; the level of BDNF in brain tissue of the rats in CAI group was increased significantly (P<0.05), and the serum BDNF level was a little higher. Compared with model group, the neurologic score of the rats in CAI group was decreased (P<0.05); the neurocytes of rats were mild edema; the levels of BDNF in brain tissue and serum showed a tendency of increasing. Conclusion CAI may has a positive function in increasing BDNF expression,and the mechanism may be related to the stress reaction of corresponding neural factors in brain tissue under ischemia injury condition.

Key words: Compound Angelica Injection, focal cerebral ischemia, brain derived neurotrophic factor, disease models,animal

中图分类号:

R285.5

汤轶波, 袁伟畅, 朱春燕, 张赛, 张玮, 张林朋, 南一楠, 牟天龙, 王旭, 杨晓敏, 何雪飞, 潘彦舒. 复方当归注射液对缺血性脑损伤大鼠BDNF表达的影响[J]. 吉林大学学报(医学版), 2015, 41(06): 1103-1106.

TANG Yibo, YUAN Weichang, ZHU Chunyan, ZHANG Sai, ZHANG Wei, ZHANG Linpeng, NAN Yinan, MU Tianlong, WANG Xu, YANG Xiaomin, HE Xuefei, PAN Yanshu. Effect of Compound Angelica Injection on expression of brain derived neurotrophic factor of rats with cerebral ischemia injury[J]. Journal of Jilin University Medicine Edition, 2015, 41(06): 1103-1106.

参考文献

[1] 王达,柳志文,章乐怡,等. 复方当归注射液对肌成纤维细胞增殖和凋亡影响的研究[J]. 中南药学,2014, 12(8):775-778.

[2] 邱超,郄增旺,程凤宽,等. 复方当归注射液穴位注射配合刺络法治疗慢阻肺临床疗效及肺循环功能的观察[J]. 河北中医药学报,2011,26(1):25-26,37.

[3] 王琛,姚永琴. 阿是穴注射复方当归注射液治疗宫颈糜烂46例[J]. 社区医学杂志,2011,9(19):50-51.

[4] 王玉石,高巧云,张正标,等. 早期局部注射糖皮质激素及复方当归注射液预防老年带状疱疹后遗神经痛的疗效分析[J].中西医结合心血管病杂志,2015,3(9):117-118.

[5] 冉亚军,陈继红. 复方当归注射液对 H₂O₂所致心肌细胞凋亡的保护作用[J]. 中国中医基础医学杂志, 2012,18(8):845-846.

[6] 汤轶波,朱春燕,袁伟畅,等. 复方当归注射液对大鼠缺血性脑损伤模型的影响[J].中国中医药信息杂志,2015,22(2):68-70.

[7] 钱海兵,黄勇其,纪刚. 复方当归注射液对脑缺血再灌注大鼠脑保护作用及对内皮素-1、白介素-6 影响的实验研究[J]. 贵阳中医学院学报,2012,34(5):233-236.

[8] Longa EZ, Weinstein PR, Carlson S, et al. Reverible middle cerebral artery occlusion without cranjectomy in rats [J]. Stroke, 1989, 20(1):84-91.

[9] Lin S, Rhodes PG, Cai Z, et al. Whole body hypothermia broadens the therapeutic window of intranasally administered IGF-1 in a neonatal rat model of cerebral hypoxia-ischemia [J]. Brain Res, 2011,1385:246-256.

[10] Xia Y, Wang CZ, Liu J, et al. Brain-derived neurotrophic factor prevents phencyclidine-induced apoptosis in developing brain by parallel activation of both the ERK and PI-3K/Akt pathways[J]. Neuropharmacology, 2010,58(2):330-336.

[11] Larpthaveesarp A, Ferriero DM, Gonzalez FF. Growth factors for the treatment of ischemic brain injury (growth factor treatment) [J]. Brain Sci, 2015,5(2):165-177.

[12] Wang J, Tang Y, Zhang W, et al. Insulin-like growth factor-1 secreted by brain microvascular endothelial cells attenuates neuron injury upon ischemia [J]. FEBS J, 2013,280(15):3658-3668.

[13] 尹艳艳,李卫平,李维祖,等. 黄芪总苷对大鼠局灶性脑缺血/再灌注损伤后BDNF, TrkB和p75NTRmRNA表达的影响[J]. 中国药理学通报,2009,25 (5):672-676.

[14] 陈玲,韩江全,李文锦. 不同剂量川芎嗪和葛根素配伍对脑缺血大鼠神经元凋亡的影响[J]. 成都医学院学报, 2015,10(3):276-280.

[15] Cao YL, Chen CF, Wang AW, et al. Changes of peripheral-type benzodiazepine receptors in the penumbra area after cerebral ischemia-reperfusion injury and effects of astragaloside Ⅳ on rats [J]. Genet Mol Res, 2015,14(1):277-285.

[16] Zhou F, Wang L, Liu P, et al. Puerarin protects brain tissue against cerebral ischemia/reperfusion injury by inhibiting the inflammatory response [J]. Neural Regen Res, 2014,9(23):2074-2080.

[17] Wang N, Zhang Y, Wu L, et al. Puerarin protected the brain from cerebral ischemia injury via astrocyte apoptosis inhibition [J]. Neuropharmacology, 2014,79:282-289.

[18] 马贤德,王守岩,张威.眼针对脑缺血再灌注模型大鼠脑组织 BDNF、p75NTR表达影响及其脑保护作用机制[J]. 北京中医药大学学报,2014,37(1):48-52.

[19] 田甜,车念聪,龙建飞,等. 大脑中动脉栓塞大鼠缺血半暗区神经可塑性蛋白及神经因子的表达[J]. 中国神经精神疾病杂志,2015,41(1):15-19.

复方当归注射液对缺血性脑损伤大鼠BDNF表达的影响

Effect of Compound Angelica Injection on expression of brain derived neurotrophic factor of rats with cerebral ischemia injury

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