吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (01): 130-133.doi: 10.13481/j.1671-587x.20160126

• 临床研究 • 上一篇    下一篇

NLRP3、AIM2和CASP1基因在慢性阻塞性肺疾病急性加重发病中的作用

王国强1, 牟佳1, 李元2, 袁语泽3, 王昱博1, 宋晨雪1, 谢婧书1, 郑敬彤1, 王放1   

  1. 1. 吉林大学基础医学院病原生物学教研室, 吉林长春 130021;
    2. 同济大学口腔医学院, 上海 200092;
    3. 延边大学农学院植物系, 吉林延吉 133002
  • 收稿日期:2015-04-10 发布日期:2016-01-26
  • 通讯作者: 王放,教授,博士研究生导师(Tel:0431-85619574,E-mail:wf@jlu.edu.cn) E-mail:wf@jlu.edu.cn
  • 作者简介:王国强(1991-),男,山西省晋中市人,在读医学硕士,主要从事微生物与呼吸道疾病方面的研究。
  • 基金资助:

    吉林省科技厅科技发展计划-科技创新人才培育计划项目资助课题(20150519015JH); 吉林大学大学生创新创业训练计划国家级项目资助课题(2014A79348)

Influence of NLRP3,AIM2 and CASP1 genes in acute exacerbations of chronic obstructive pulmonary diseases

WANG Guoqiang1, MU Jia1, LI Yuan2, YUAN Yuze3, WANG Yubo1, SONG Chenxue1, XIE Jingshu1, ZHENG Jingtong1, WANG Fang1   

  1. 1. Department of Pathogen Biology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China;
    2. School of Stomatology, Tongji University, Shanghai 200092, China;
    3. Department of Botany, College of Agriculture, Yanbian University, Yanji 133002, China
  • Received:2015-04-10 Published:2016-01-26

摘要:

目的: 探讨固有免疫中核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)、黑素瘤缺乏因子2(AIM2)、半胱天冬酶1(CASP1)基因和白细胞介素1β(IL-1β)、白细胞介素18(IL-18)炎症因子在慢性阻塞性肺疾病(COPD)急性发作期(AECOPD)患者体内的表达情况,阐明其作用机制。 方法: 收集健康对照组(12例)和AECOPD患者(20例)的痰液,分别进行痰处理,采用实时荧光定量PCR法检测NLRP3、AIM2和CASP1基因表达水平,通过ELISA法检测痰上清中的IL-1β和IL-18蛋白表达水平。 结果: 与健康对照组比较,AECOPD组患者NLRP3、AIM2和CASP1基因表达分别上调3.83、1.70和2.42 倍,NLRP3、AIM2和CASP1基因表达水平明显增高(P<0.01),AECOPD组患者痰上清中IL-1β和IL-18蛋白表达水平明显增高(P<0.05)。 结论: NLRP3及AIM2炎性体参与AECOPD的病理过程,其作用机制可能与固有免疫活化有关。

关键词: 慢性阻塞性肺疾病急性发作期, 核苷酸结合寡聚化结构域样受体蛋白3, 黑素瘤缺乏因子2, 半胱天冬酶1, 固有免疫

Abstract:

Objective: To study the expressions of NLRP3,AIM2,CASP1 genes and interleukin-1β (IL-1β),interleukin-18 (IL-18)in the patients with acute exacerbations of chronic obstructive pulmonary diseases (AECOPD),and to clarify their mechanisms.Methods: The sputum of patients with AECOPD (n=20)and controls (n=12)were collected,the expressions of NLRP3,AIM2 and CASP1 genes were determined by Real-Time fluorescent quantitative PCR in two groups. and the expressions of IL-1β and IL-18 in sputum supernatant were detected by ELISA. Results: Compared with control group,the expressions of NLRP3,AIM2 and CASP1 genes of the patients in AECOPD group were elevated by 3.83,1.70,2.42 times,respectively (P<0.05);the expression levels of IL-1β and IL-18 in sputum supernatant of the patients in AECOPD were increased significantly (P < 0.05).Conclusion: NLRP3 and AIM2 genes are involved in the pathological process of AECOPD.

Key words: acute exacerbation chronic obstructive pulmonary disease, NOD-like receptor protein 3, absent in melanoma 2, cysteinyl aspartate specific proteinase-1, innate immune

中图分类号: 

  • R563